ArticleApplied and environmental microbiology2013
DNA double-strand break repair at--15{degrees}C.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The Taxonomic Diversity of Prokaryotic Communities from Permafrost Active Layers of the Chilean Andes.Microorganisms · 2026Article
- Review
- HREM, RNAseq and Cell Cycle Analyses Reveal the Role of the G2/M-Regulatory Protein, WEE1, on the Survivability of Chicken Embryos during Diapause.Biomedicines · 2022Article
- Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost.Microbiome · 2021Article
- Taxonomic and functional characterization of a microbial community from a volcanic englacial ecosystem in Deception Island, Antarctica.Scientific reports · 2019Article
- Predominance of Anaerobic, Spore-Forming Bacteria in Metabolically Active Microbial Communities from Ancient Siberian Permafrost.Applied and environmental microbiology · 2019Article
- Microbial activity in Martian analog soils after ionizing radiation: implications for the preservation of subsurface life on Mars.AIMS microbiology · 2018Article
- Evolution with a seed bank: The population genetic consequences of microbial dormancy.Evolutionary applications · 2018Review
- 100 kGy gamma-affected microbial communities within the ancient Arctic permafrost under simulated Martian conditions.Extremophiles : life under extreme conditions · 2017Article
- Microbial survival strategies in ancient permafrost: insights from metagenomics.The ISME journal · 2017Article
- Glaciers and Ice Sheets As Analog Environments of Potentially Habitable Icy Worlds.Frontiers in microbiology · 2017Review
- The transcriptional response of microbial communities in thawing Alaskan permafrost soils.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The survival of microorganisms in ancient glacial ice and permafrost has been ascribed to their ability to persist in a dormant, metabolically inert state. An alternative possibility, supported by experimental data, is that microorganisms in frozen matrices are able to sustain a level of metabolic function that is sufficient for cellular repair and maintenance. To examine this experimentally, frozen populations of Psychrobacter arcticus 273-4 were exposed to ionizing radiation (IR) to simulate the damage incurred from natural background IR sources in the permafrost environment from over ∼225 kiloyears (ky). High-molecular-weight DNA was fragmented by exposure to 450 Gy of IR, which introduced an average of 16 double-strand breaks (DSBs) per chromosome. During incubation at -15°C for 505 days, P. arcticus repaired DNA DSBs in the absence of net growth. Based on the time frame for the assembly of genomic fragments by P. arcticus, the rate of DNA DSB repair was estimated at 7 to 10 DSBs year(-1) under the conditions tested. Our results provide direct evidence for the repair of DNA lesions, extending the range of complex biochemical reactions known to occur in bacteria at frozen temperatures. Provided that sufficient energy and nutrient sources are available, a functional DNA repair mechanism would allow cells to maintain genome integrity and augment microbial survival in icy terrestrial or extraterrestrial environments.
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