ArticleJournal of biomedical optics2014
Thin-film tunable filters for hyperspectral fluorescence microscopy.
Article in Journal of biomedical optics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications.Journal of biomedical optics · 2026Pooled it
- High-speed hyperspectral single-pixel microscopy via line-scan detection with data fusion-based enhanced resolution.Communications engineering · 2026Article
- A Review of Recent Advances in Computer-Aided Detection Methods Using Hyperspectral Imaging Engineering to Detect Skin Cancer.Cancers · 2023Review
- Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus.Nature communications · 2023Article
- Comparing Performance of Spectral Image Analysis Approaches for Detection of Cellular Signals in Time-Lapse Hyperspectral Imaging Fluorescence Excitation-Scanning Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
- Microscopy is better in color: development of a streamlined spectral light path for real-time multiplex fluorescence microscopy.Biomedical optics express · 2022Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- Electrostatically Tuned Optical Filters Based on Hybrid Plasmonic-Dielectric Thin Films for Hyperspectral Imaging.Micromachines · 2021Article
- Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing.Light, science & applications · 2021Article
- Hyperspectral and multispectral imaging in digital and computational pathology: a systematic review [Invited].Biomedical optics express · 2020Review
- A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections.Journal of visualized experiments : JoVE · 2020Article
- Comparison of spectral FRET microscopy approaches for single-cell analysis.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Ratiometric widefield imaging with spectrally balanced detection.Biomedical optics express · 2019Article
- Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals.Journal of visualized experiments : JoVE · 2019Article
- Information theoretic analysis of hyperspectral imaging systems with applications to fluorescence microscopy.Biomedical optics express · 2019Article
- Sensitivity analysis of a multibranched light guide for real time hyperspectral imaging systems.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Optimization of Light Transmission through an Excitation-scan Hyperspectral Mirror Array System.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Hyperspectral imaging fluorescence excitation scanning (HIFEX) microscopy for live cell imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Identifying molecular contributors to autofluorescence of neoplastic and normal colon sections using excitation-scanning hyperspectral imaging.Journal of biomedical optics · 2018Article
- Spectral imaging of FRET-based sensors reveals sustained cAMP gradients in three spatial dimensions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Hyperspectral imaging is a powerful tool that acquires data from many spectral bands, forming a contiguous spectrum. Hyperspectral imaging was originally developed for remote sensing applications; however, hyperspectral techniques have since been applied to biological fluorescence imaging applications, such as fluorescence microscopy and small animal fluorescence imaging. The spectral filtering method largely determines the sensitivity and specificity of any hyperspectral imaging system. There are several types of spectral filtering hardware available for microscopy systems, most commonly acousto-optic tunable filters (AOTFs) and liquid crystal tunable filters (LCTFs). These filtering technologies have advantages and disadvantages. Here, we present a novel tunable filter for hyperspectral imaging-the thin-film tunable filter (TFTF). The TFTF presents several advantages over AOTFs and LCTFs, most notably, a high percentage transmission and a high out-of-band optical density (OD). We present a comparison of a TFTF-based hyperspectral microscopy system and a commercially available AOTF-based system. We have characterized the light transmission, wavelength calibration, and OD of both systems, and have then evaluated the capability of each system for discriminating between green fluorescent protein and highly autofluorescent lung tissue. Our results suggest that TFTFs are an alternative approach for hyperspectral filtering that offers improved transmission and out-of-band blocking. These characteristics make TFTFs well suited for other biomedical imaging devices, such as ophthalmoscopes or endoscopes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.