ArticleGenetics2013
Adaptive divergence in experimental populations of Pseudomonas fluorescens. V. Insight into the niche specialist fuzzy spreader compels revision of the model Pseudomonas radiation.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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29 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Integrating pH into the metabolic theory of ecology to predict bacterial diversity in soil.Proceedings of the National Academy of Sciences of the United States of America · 2023Pooled it
- Genetic diversification ofApplied and environmental microbiology · 2026Article
- GnT Motifs Can Increase T:A→G:C Mutation Rates Over 1000-fold in Bacteria.Molecular biology and evolution · 2025Article
- Student-led experimental evolution reveals novel biofilm regulators of adaptation to multiple niches.bioRxiv : the preprint server for biology · 2025Article
- Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens.Environmental microbiology · 2025Article
- Distribution of mutation rates challenges evolutionary predictability.Microbiology (Reading, England) · 2023Article
- Public good-driven release of heterogeneous resources leads to genotypic diversification of an isogenic yeast population.Evolution; international journal of organic evolution · 2022Article
- Adaptation and phenotypic diversification of Bacillus thuringiensis biofilm are accompanied by fuzzy spreader morphotypes.NPJ biofilms and microbiomes · 2022Article
- Transposable elements promote the evolution of genome streamlining.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
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- Forecasting of phenotypic and genetic outcomes of experimental evolution in Pseudomonas protegens.PLoS genetics · 2021Article
- The evolution of coexistence from competition in experimental co-cultures of Escherichia coli and Saccharomyces cerevisiae.The ISME journal · 2021Article
- Identification of a Transcriptomic Network Underlying the Wrinkly and Smooth Phenotypes of Vibrio fischeri.Journal of bacteriology · 2021Article
- CRISPR interference to interrogate genes that control biofilm formation in Pseudomonas fluorescens.Scientific reports · 2019Article
- Article
- Article
- Complete Genome Sequences of Three NovelGenome announcements · 2017Article
- Spatial storage effect promotes biodiversity during adaptive radiation.Proceedings. Biological sciences · 2017Article
- Evolutionary convergence in experimental Pseudomonas populations.The ISME journal · 2017Article
- Transposon insertion libraries for the characterization of mutants from the kiwifruit pathogen Pseudomonas syringae pv. actinidiae.PloS one · 2017Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas fluorescens is a model for the study of adaptive radiation. When propagated in a spatially structured environment, the bacterium rapidly diversifies into a range of niche specialist genotypes. Here we present a genetic dissection and phenotypic characterization of the fuzzy spreader (FS) morphotype-a type that arises repeatedly during the course of the P. fluorescens radiation and appears to colonize the bottom of static broth microcosms. The causal mutation is located within gene fuzY (pflu0478)-the fourth gene of the five-gene fuzVWXYZ operon. fuzY encodes a β-glycosyltransferase that is predicted to modify lipopolysaccharide (LPS) O antigens. The effect of the mutation is to cause cell flocculation. Analysis of 92 independent FS genotypes showed each to have arisen as the result of a loss-of-function mutation in fuzY, although different mutations have subtly different phenotypic and fitness effects. Mutations within fuzY were previously shown to suppress the phenotype of mat-forming wrinkly spreader (WS) types. This prompted a reinvestigation of FS niche preference. Time-lapse photography showed that FS colonizes the meniscus of broth microcosms, forming cellular rafts that, being too flimsy to form a mat, collapse to the vial bottom and then repeatably reform only to collapse. This led to a reassessment of the ecology of the P. fluorescens radiation. Finally, we show that ecological interactions between the three dominant emergent types (smooth, WS, and FS), combined with the interdependence of FS and WS on fuzY, can, at least in part, underpin an evolutionary arms race with bacteriophage SBW25Φ2, to which mutation in fuzY confers resistance.
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