ArticleThe Plant cell2013
Dual role for phospholipid:diacylglycerol acyltransferase: enhancing fatty acid synthesis and diverting fatty acids from membrane lipids to triacylglycerol in Arabidopsis leaves.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 176 citations in OpenAlex.
- Disruption of starch biosynthesis and triacylglycerol degradation impairs growth but improves photosynthesis in Arabidopsis.The New phytologist · 2026Article
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- Characterization ofPlants (Basel, Switzerland) · 2025Article
- A comprehensive evaluation of metabolically engineered Cynara cardunculus calli as platform for valuable fatty acid derivatives production.Scientific reports · 2025Article
- Enhanced seed oil content by overexpressing LPAAT and WRI1 genes in tobacco plant.Plant cell reports · 2025Article
- Inducible expression of DEFECTIVE IN ANTHER DEHISCENCE 1 enhances triacylglycerol accumulation and lipid droplet formation in vegetative tissues.The Plant journal : for cell and molecular biology · 2025Article
- Metabolic flux analysis to increase oil in seeds.Plant physiology · 2025Review
- Synergistic mechanisms of DGAT and PDAT in shaping triacylglycerol diversity: evolutionary insights and metabolic engineering strategies.Frontiers in plant science · 2025Review
- Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism.BMC plant biology · 2024Article
- Transcriptome analysis of the common mossAoB PLANTS · 2024Article
- Does periphyton turn less palatable under grazing pressure?ISME communications · 2024Article
- Glycerol-3-Phosphate Acyltransferase GPAT9 Enhanced Seed Oil Accumulation and Eukaryotic Galactolipid Synthesis inInternational journal of molecular sciences · 2023Article
- Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues.Biotechnology for biofuels and bioproducts · 2023Article
- Phospholipid:diacylglycerol acyltransferase1-overexpression stimulates lipid turnover, oil production and fitness in cold-grown plants.BMC plant biology · 2023Article
- The ubiquitin-protein ligase MIEL1 localizes to peroxisomes to promote seedling oleosin degradation and lipid droplet mobilization.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Dynamic Regulation of Lipid Droplet Biogenesis in Plant Cells and Proteins Involved in the Process.International journal of molecular sciences · 2023Review
- Progress in understanding and improving oil content and quality in seeds.Frontiers in plant science · 2023Review
- Article
- Bioengineering to Accelerate Biodiesel Production for a Sustainable Biorefinery.Bioengineering (Basel, Switzerland) · 2022Review
- Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass.BMC biotechnology · 2022Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is growing interest in engineering green biomass to expand the production of plant oils as feed and biofuels. Here, we show that phospholipid:diacylglycerol acyltransferase1 (PDAT1) is a critical enzyme involved in triacylglycerol (TAG) synthesis in leaves. Overexpression of PDAT1 increases leaf TAG accumulation, leading to oil droplet overexpansion through fusion. Ectopic expression of oleosin promotes the clustering of small oil droplets. Coexpression of PDAT1 with oleosin boosts leaf TAG content by up to 6.4% of the dry weight without affecting membrane lipid composition and plant growth. PDAT1 overexpression stimulates fatty acid synthesis (FAS) and increases fatty acid flux toward the prokaryotic glycerolipid pathway. In the trigalactosyldiacylglycerol1-1 mutant, which is defective in eukaryotic thylakoid lipid synthesis, the combined overexpression of PDAT1 with oleosin increases leaf TAG content to 8.6% of the dry weight and total leaf lipid by fourfold. In the plastidic glycerol-3-phosphate acyltransferase1 mutant, which is defective in the prokaryotic glycerolipid pathway, PDAT1 overexpression enhances TAG content at the expense of thylakoid membrane lipids, leading to defects in chloroplast division and thylakoid biogenesis. Collectively, these results reveal a dual role for PDAT1 in enhancing fatty acid and TAG synthesis in leaves and suggest that increasing FAS is the key to engineering high levels of TAG accumulation in green biomass.
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