ArticleCurrent biology : CB2013
A 3D map of the yeast kinetochore reveals the presence of core and accessory centromere-specific histone.
Article in Current biology : CB, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 56 citations in OpenAlex.
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- The centromere bottlebrush requires a multi-microtubule attachment.Molecular biology of the cell · 2025Article
- Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.Molecular biology of the cell · 2023Article
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Nanoscale structural organization and stoichiometry of the budding yeast kinetochore.The Journal of cell biology · 2023Article
- Polymer models reveal how chromatin modification can modulate force at the kinetochore.Molecular biology of the cell · 2022Article
- Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.Molecular biology of the cell · 2021Article
- R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast.Molecular biology of the cell · 2021Article
- ImageJ for Partially and Fully Automated Analysis of Trypanosome Micrographs.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Protein kinases in mitotic phosphorylation of budding yeast CENP-A.Current genetics · 2019Review
- Article
- The regulation of chromosome segregation via centromere loops.Critical reviews in biochemistry and molecular biology · 2019Review
- Article
- The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.The Journal of cell biology · 2019Article
- Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast.Chromosoma · 2019Article
- Cell cycle-dependent association of polo kinase Cdc5 with CENP-A contributes to faithful chromosome segregation in budding yeast.Molecular biology of the cell · 2019Article
- AI-Assisted Forward Modeling of Biological Structures.Frontiers in cell and developmental biology · 2019Article
- Conformational flexibility of histone variant CENP-AThe Journal of biological chemistry · 2018Article
- A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation.Current biology : CB · 2018Article
- Five pillars of centromeric chromatin in fungal pathogens.PLoS pathogens · 2018Review
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9 authors at 2 institutions in 1 country.
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Abstract
The budding yeast kinetochore is ~68 nm in length with a diameter slightly larger than a 25 nm microtubule. The kinetochores from the 16 chromosomes are organized in a stereotypic cluster encircling central spindle microtubules. Quantitative analysis of the inner kinetochore cluster (Cse4, COMA) reveals structural features not apparent in singly attached kinetochores. The cluster of Cse4-containing kinetochores is physically larger perpendicular to the spindle axis relative to the cluster of Ndc80 molecules. If there was a single Cse4 (molecule or nucleosome) at the kinetochore attached to each microtubule plus end, the cluster of Cse4 would appear geometrically identical to Ndc80. Thus, the structure of the inner kinetochore at the surface of the chromosomes remains unsolved. We have used point fluorescence microscopy and statistical probability maps to deduce the two-dimensional mean position of representative components of the yeast kinetochore relative to the mitotic spindle in metaphase. Comparison of the experimental images to three-dimensional architectures from convolution of mathematical models reveals a pool of Cse4 radially displaced from Cse4 at the kinetochore and kinetochore microtubule plus ends. The pool of displaced Cse4 can be experimentally depleted in mRNA processing pat1Δ or xrn1Δ mutants. The peripheral Cse4 molecules do not template outer kinetochore components. This study suggests an inner kinetochore plate at the centromere-microtubule interface in budding yeast and yields information on the number of Ndc80 molecules at the microtubule attachment site.
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