ArticleDevelopmental cell2013
Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly.
Article in Developmental cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Article
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- IMPACTS OF DNA METHYLATION ON H2A.Z DEPOSITION AND NUCLEOSOME STABILITY.bioRxiv : the preprint server for biology · 2025Article
- Microtubules Disruption Alters the Cellular Structures and Mechanics Depending on Underlying Chemical Cues.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Article
- Spastin regulates anaphase chromosome separation distance and microtubule-containing nuclear tunnels.Molecular biology of the cell · 2024Article
- Regulation of organelle size and organization during development.Seminars in cell & developmental biology · 2023Review
- Hubbing the Cancer Cell.Cancers · 2022Review
- Size regulation of multiple organelles competing for a limiting subunit pool.PLoS computational biology · 2022Article
- Nuclear Morphological Abnormalities in Cancer: A Search for Unifying Mechanisms.Results and problems in cell differentiation · 2022Article
- An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors.Cell reports · 2021Article
- Review
- Article
- The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.Communications biology · 2021Article
- The coordination of nuclear envelope assembly and chromosome segregation in metazoans.Nucleus (Austin, Tex.) · 2020Review
- Small but mighty: the causes and consequences of micronucleus rupture.Experimental & molecular medicine · 2020Review
- Non-Canonical Functions of the Gamma-Tubulin Meshwork in the Regulation of the Nuclear Architecture.Cancers · 2020Review
- Organelle size scaling over embryonic development.Wiley interdisciplinary reviews. Developmental biology · 2020Review
- Mechanical stress triggers nuclear remodeling and the formation of transmembrane actin nuclear lines with associated nuclear pore complexes.Molecular biology of the cell · 2020Article
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Abstract
Nuclear shape and size vary between species, during development, and in many tissue pathologies, but the causes and effects of these differences remain poorly understood. During fertilization, sperm nuclei undergo a dramatic conversion from a heavily compacted form into decondensed, spherical pronuclei, accompanied by rapid nucleation of microtubules from centrosomes. Here we report that the assembly of the spherical nucleus depends on a critical balance of microtubule dynamics, which is regulated by the chromatin-binding protein Developmental pluripotency-associated 2 (Dppa2). Whereas microtubules normally promote sperm pronuclear expansion, in Dppa2-depleted Xenopus egg extracts excess microtubules cause pronuclear assembly defects, leading to abnormal morphology and disorganized DNA replication. Dppa2 inhibits microtubule polymerization in vitro, and Dppa2 activity is needed at a precise time and location during nascent pronuclear formation. This demonstrates a strict spatiotemporal requirement for local suppression of microtubules during nuclear formation, fulfilled by chromatin-bound microtubule regulators.
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