Evidence map›Paper›PMID 24075410›Full record

ArticleBiomaterials2013

Porous chitosan-hyaluronic acid scaffolds as a mimic of glioblastoma microenvironment ECM.

Stephen J Florczyk, Kui Wang, Soumen Jana, David L Wood, Samara K Sytsma, Jonathan Sham, Forrest M Kievit, Miqin Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.

0numbers the graph read from it
0cells of the map it votes in
87citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

87 citing papers in PubMed, 215 citations in OpenAlex.

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  15. Biomaterial-basedCancer pathogenesis and therapy · 2023
    Review
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  18. Article
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27 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Stephen J Florczyk *Department of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
Kui Wang *Department of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
Soumen JanaDepartment of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
David L WoodDepartment of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
Samara K SytsmaDepartment of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
Jonathan ShamDepartment of Surgery, University of Washington, Seattle, WA, 98195, USA.
Forrest M KievitDepartment of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
Miqin ZhangDepartment of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.
University of Washington · US

Funding

Nanotechnology and Physical Science Training Program in Cancer ResearchT32CA138312 · NCI · UNIVERSITY OF WASHINGTON · PI ZHANG, MIQIN · 2009 to 2013
$1.5M
NCI NIH HHS T32 CA138312NCI NIH HHS T32CA138312
6 · The paper itself

Abstract

Cancer therapeutics are developed through extensive screening; however, many therapeutics evaluated with 2D in vitro cultures during pre-clinical trials suffer from lower efficacy in patients. Replicating the in vivo tumor microenvironment in vitro with three-dimensional (3D) porous scaffolds offers the possibility of generating more predictive pre-clinical models to enhance cancer treatment efficacy. We developed a chitosan and hyaluronic acid (HA) polyelectrolyte complex 3D porous scaffold and evaluated its physical properties. Chitosan-HA (C-HA) scaffolds had a highly porous network. C-HA scaffolds were compared to 2D surfaces for in vitro culture of U-118 MG human glioblastoma (GBM) cells. C-HA scaffold cultures promoted tumor spheroid formation and increased stem-like properties of GBM cells as evidenced by the upregulation of CD44, Nestin, Musashi-1, GFAP, and HIF-1α as compared with 2D cultures. Additionally, the invasiveness of GBM cells cultured in C-HA scaffolds was significantly enhanced compared to those grown in 2D cultures. C-HA scaffold cultures were also more resistant to chemotherapy drugs, which corresponded to the increased expression of ABCG2 drug efflux transporter. These findings suggest that C-HA scaffolds offer promise as an in vitro GBM platform for study and screening of novel cancer therapeutics.

Indexed as

Cell Line, TumorCell ProliferationChitosanExtracellular MatrixGlioblastomaHumansHyaluronic AcidMicroscopy, Electron, ScanningPorositySpectroscopy, Fourier Transform InfraredTissue ScaffoldsChitosanHyaluronic AcidCancer stem cellGliomaInvasionMultidrug resistanceSpheroid

Identifiers

PMID24075410
PMCPMC3843957
OpenAlexW2097730243

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.