ArticleVirology2013
Development of a reverse genetics system to generate recombinant Marburg virus derived from a bat isolate.
Article in Virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Exploratory Temporal and Evolutionary Insights into theMicroorganisms · 2025Article
- Marburg Virus Minigenome Assays.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Recovery of Recombinant Marburg Virus by Reverse Genetics.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Marburg virus exploits the Rab11-mediated endocytic pathway in viral-particle production.Microbiology spectrum · 2024Article
- Review
- Development of a Měnglà virus minigenome and comparison of its polymerase complexes with those of other filoviruses.Virologica Sinica · 2024Article
- Human macrophages infected with Egyptian Rousette bat-isolated Marburg virus display inter-individual susceptibility and antiviral responsiveness.Npj viruses · 2024Article
- Fluorescent and Bioluminescent Reporter Mouse-Adapted Ebola Viruses Maintain Pathogenicity and Can Be Visualized in Vivo.The Journal of infectious diseases · 2023Article
- Marburg and Ebola Virus Infections Elicit a Complex, Muted Inflammatory State in Bats.Viruses · 2023Article
- Ebola Virus Produces Discrete Small Noncoding RNAs Independently of the Host MicroRNA Pathway Which Lack RNA Interference Activity in Bat and Human Cells.Journal of virology · 2020Article
- Distinct Genome Replication and Transcription Strategies within the Growing Filovirus Family.Journal of molecular biology · 2019Review
- Pulmonary Involvement during the Ebola Virus Disease.Viruses · 2019Review
- Article
- Recent advances in marburgvirus research.F1000Research · 2019Review
- Article
- Phosphorylated VP30 of Marburg Virus Is a Repressor of TranscriptionJournal of virology · 2018Article
- A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses.Cell reports · 2018Article
- Post-exposure treatments for Ebola and Marburg virus infections.Nature reviews. Drug discovery · 2018Review
- Transcriptional analysis of viral mRNAs reveals common transcription patterns in cells infected by five different filoviruses.PloS one · 2018Article
- 4'-Azidocytidine (R1479) inhibits henipaviruses and other paramyxoviruses with high potency.Antiviral research · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Recent investigations have shown the Egyptian fruit bat (Rousettus aegyptiacus) to be a natural reservoir for marburgviruses. To better understand the life cycle of these viruses in the natural host, a new reverse genetics system was developed for the reliable rescue of a Marburg virus (MARV) originally isolated directly from a R. aegyptiacus bat (371Bat). To develop this system, the exact terminal sequences were first determined by 5' and 3' RACE, followed by the cloning of viral proteins NP, VP35, VP30 and L into expression plasmids. Novel conditions were then developed to efficiently replicate virus mini-genomes followed by the construction of full-length genomic clones from which recombinant wild type and GFP-containing MARVs were rescued. Surprisingly, when these recombinant MARVs were propagated in primary human macrophages, a dramatic difference was found in their ability to grow and to elicit anti-viral cytokine responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.