ArticleStem cells and development2014
Genome wide profiling of dopaminergic neurons derived from human embryonic and induced pluripotent stem cells.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Novel human pluripotent stem cell-derived hypothalamus organoids demonstrate cellular diversity.iScience · 2023Article
- Applications of Boolean modeling to study the dynamics of a complex disease and therapeutics responses.Frontiers in bioinformatics · 2023Article
- High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells.STAR protocols · 2021Article
- Article
- A single-cell Raman-based platform to identify developmental stages of human pluripotent stem cell-derived neurons.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Parkinson's disease, aging and adult neurogenesis: Wnt/β-catenin signalling as the key to unlock the mystery of endogenous brain repair.Aging cell · 2020Review
- Crosstalk of Intercellular Signaling Pathways in the Generation of Midbrain Dopaminergic Neurons In Vivo and from Stem Cells.Journal of developmental biology · 2019Review
- Wnt/β-Catenin Signaling Pathway Governs a Full Program for Dopaminergic Neuron Survival, Neurorescue and Regeneration in the MPTP Mouse Model of Parkinson's Disease.International journal of molecular sciences · 2018Review
- Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.Frontiers in medicine · 2018Article
- Altered Differentiation Potential of Gaucher's Disease iPSC Neuronal Progenitors due to Wnt/β-Catenin Downregulation.Stem cell reports · 2017Article
- Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications.Stem cell reviews and reports · 2016Article
- Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes.Brain research · 2016Article
- Comparison of Human Primary with Human iPS Cell-Derived Dopaminergic Neuron Grafts in the Rat Model for Parkinson's Disease.Stem cell reviews and reports · 2016Article
- Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells.Frontiers in cellular neuroscience · 2016Article
- Article
- Genome-wide assessment of differential effector gene use in embryogenesis.Development (Cambridge, England) · 2015Article
- cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications.Stem cell reports · 2015Article
- Quantifying signaling pathway activation to monitor the quality of induced pluripotent stem cells.Oncotarget · 2015Article
- Mitochondrial alterations by PARKIN in dopaminergic neurons using PARK2 patient-specific and PARK2 knockout isogenic iPSC lines.Stem cell reports · 2015Article
- Molecular mechanisms of dopaminergic subset specification: fundamental aspects and clinical perspectives.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) biology enable generation of dopaminergic neurons for potential therapy and drug screening. However, our current understanding of molecular and cellular signaling that controls human dopaminergic development and function is limited. Here, we report on a whole genome analysis of gene expression during dopaminergic differentiation of human ESC/iPSC using Illumina bead microarrays. We generated a transcriptome data set containing the expression levels of 28,688 unique transcripts by profiling five lines (three ESC and two iPSC lines) at four stages of differentiation: (1) undifferentiated ESC/iPSC, (2) neural stem cells, (3) dopaminergic precursors, and (4) dopaminergic neurons. This data set provides comprehensive information about genes expressed at each stage of differentiation. Our data indicate that distinct pathways are activated during neural and dopaminergic neuronal differentiation. For example, WNT, sonic hedgehog (SHH), and cAMP signaling pathways were found over-represented in dopaminergic populations by gene enrichment and pathway analysis, and their role was confirmed by perturbation analyses using RNAi (small interfering RNA of SHH and WNT) or small molecule [dibutyryl cyclic AMP (dcAMP)]. In summary, whole genome profiling of dopaminergic differentiation enables systematic analysis of genes/pathways, networks, and cellular/molecular processes that control cell fate decisions. Such analyses will serve as the foundation for better understanding of dopaminergic development, function, and development of future stem cell-based therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.