ArticlePloS one2013
Novel 3D co-culture model for epithelial-stromal cells interaction in prostate cancer.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Prostate cancer cells' growth is decreased by novel MYC inhibitors.Investigational new drugs · 2026Article
- Microfluidic Applications in Prostate Cancer Research.Micromachines · 2024Review
- A novel co-culture model of human prostate epithelial and stromal cells for androgenic and antiandrogenic screening.Toxicology in vitro : an international journal published in association with BIBRA · 2023Article
- Review
- Review
- 3D-culture models as drug-testing platforms in canine lymphoma and their cross talk with lymph node-derived stromal cells.Journal of veterinary science · 2021Article
- Differential impact of paired patient-derived BPH and normal adjacent stromal cells on benign prostatic epithelial cell growth in 3D culture.The Prostate · 2020Article
- Prostate Cancer Phenotype Influences Bone Mineralization at Metastasis: A Study Using an In Vitro Prostate Cancer Metastasis Testbed.JBMR plus · 2020Article
- Recent Advances in Cancer Plasticity: Cellular Mechanisms, Surveillance Strategies, and Therapeutic Optimization.Frontiers in oncology · 2020Review
- Human stroma and epithelium co-culture in a microfluidic model of a human prostate gland.Biomicrofluidics · 2019Article
- Macrofluidic recirculating model of skeletal metastasis.Scientific reports · 2019Article
- Bio-instructive materials for musculoskeletal regeneration.Acta biomaterialia · 2019Review
- Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system.BMC biotechnology · 2019Article
- Prostate Stroma Increases the Viability and Maintains the Branching Phenotype of Human Prostate Organoids.iScience · 2019Article
- Cisplatin-Loaded Graphene Oxide/Chitosan/Hydroxyapatite Composite as a Promising Tool for Osteosarcoma-Affected Bone Regeneration.ACS omega · 2018Article
- Design of spherically structured 3D in vitro tumor models -Advances and prospects.Acta biomaterialia · 2018Review
- Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip.Global challenges (Hoboken, NJ) · 2018Article
- 3d Tissue Engineered In Vitro Models Of Cancer In Bone.ACS biomaterials science & engineering · 2018Article
- Epithelial-mesenchymal crosstalk influences cellular behavior in a 3D alveolus-fibroblast model system.Biomaterials · 2018Article
- The Microwell-mesh: A high-throughput 3D prostate cancer spheroid and drug-testing platform.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Paracrine function is a major mechanism of cell-cell communication within tissue microenvironment in normal development and disease. In vitro cell culture models simulating tissue or tumor microenvironment are necessary tools to delineate epithelial-stromal interactions including paracrine function, yet an ideal three-dimensional (3D) tumor model specifically studying paracrine function is currently lacking. In order to fill this void we developed a novel 3D co-culture model in double-layered alginate hydrogel microspheres, incorporating prostate cancer epithelial and stromal cells in separate compartments of the microspheres. The cells remained confined and viable within their respective spheres for over 30 days. As a proof of principle regarding paracrine function of the model, we measured shedded component of E-cadherin (sE-cad) in the conditioned media, a major membrane bound cell adhesive molecule that is highly dysregulated in cancers including prostate cancer. In addition to demonstrating that sE-cad can be reliably quantified in the conditioned media, the time course experiments also demonstrated that the amount of sE-cad is influenced by epithelial-stromal interaction. In conclusion, the study establishes a novel 3D in vitro co-culture model that can be used to study cell-cell paracrine interaction.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.