ArticleThe Journal of experimental biology2013
Salinity-induced regulation of the myo-inositol biosynthesis pathway in tilapia gill epithelium.
Article in The Journal of experimental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- In silico characterization and molecular docking of the MIOX gene in Nile tilapia (Oreochromis niloticus).Fish physiology and biochemistry · 2026Article
- Comparative Proteomics of Salinity Stress Responses in Fish and Aquatic Invertebrates.Proteomics · 2025Review
- Salinity-responsive histone PTMs identified in the gills and gonads of Mozambique tilapia (Oreochromis mossambicus).BMC genomics · 2024Article
- Interplay of gene expression and regulators under salinity stress in gill of Labeo rohita.BMC genomics · 2023Article
- Article
- Gliotransmission of D-serine promotes thirst-directed behaviors in Drosophila.Current biology : CB · 2022Article
- Physiological and Metabolic Responses of Gac Leaf (Plants (Basel, Switzerland) · 2022Article
- Kidney transcriptome response to salinity adaptation inFrontiers in physiology · 2022Article
- The genome of walking catfish Clarias magur (Hamilton, 1822) unveils the genetic basis that may have facilitated the development of environmental and terrestrial adaptation systems in air-breathing catfishes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- An osmolality/salinity-responsive enhancer 1 (OSRE1) in intron 1 promotes salinity induction of tilapia glutamine synthetase.Scientific reports · 2020Article
- The round goby genome provides insights into mechanisms that may facilitate biological invasions.BMC biology · 2020Article
- Osmolality/salinity-responsive enhancers (OSREs) control induction of osmoprotective genes in euryhaline fish.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus): effect of seawater acclimation.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Transcriptomic analysis reveals specific osmoregulatory adaptive responses in gill mitochondria-rich cells and pavement cells of the Japanese eel.BMC genomics · 2015Article
- Direct Ionic Regulation of the Activity of Myo-Inositol Biosynthesis Enzymes in Mozambique Tilapia.PloS one · 2015Article
- Article
- Derivation and osmotolerance characterization of three immortalized tilapia (Oreochromis mossambicus) cell lines.PloS one · 2014Article
- Tilapia (Oreochromis mossambicus) brain cells respond to hyperosmotic challenge by inducing myo-inositol biosynthesis.The Journal of experimental biology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The myo-inositol biosynthesis (MIB) pathway converts glucose-6-phosphate to the compatible osmolyte myo-inositol that protects cells from osmotic stress. Using proteomics, the enzymes that constitute the MIB pathway, myo-inositol phosphate synthase (MIPS) and inositol monophosphatase 1 (IMPA1), are identified in tilapia (Oreochromis mossambicus) gill epithelium. Targeted, quantitative, label-free proteomics reveals that they are both upregulated during salinity stress. Upregulation is stronger when fish are exposed to severe (34 ppt acute and 90 ppt gradual) relative to moderate (70 ppt gradual) salinity stress. IMPA1 always responds more strongly than MIPS, suggesting that MIPS is more stable during salinity stress. MIPS is N-terminally acetylated and the corresponding peptide increases proportionally to MIPS protein, while non-acetylated N-terminal peptide is not detectable, indicating that MIPS acetylation is constitutive and may serve to stabilize the protein. Hyperosmotic induction of MIPS and IMPA1 is confirmed using western blot and real-time qPCR and is much higher at the mRNA than at the protein level. Two distinct MIPS mRNA variants are expressed in the gill, but one is more strongly regulated by salinity than the other. A single MIPS gene is encoded in the tilapia genome whereas the zebrafish genome lacks MIPS entirely. The genome of euryhaline tilapia contains four IMPA genes, two of which are expressed, but only one is salinity regulated in gill epithelium. The genome of stenohaline zebrafish contains a single IMPA gene. We conclude that the MIB pathway represents a major salinity stress coping mechanism that is regulated at multiple levels in euryhaline fish but absent in stenohaline zebrafish.
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