ArticleStem cell reviews and reports2014
Reprogramming of pig dermal fibroblast into insulin secreting cells by a brief exposure to 5-aza-cytidine.
Article in Stem cell reviews and reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Stem Cells Reprogramming in Diabetes Mellitus and Diabetic Complications: Recent Advances.Current diabetes reviews · 2025Review
- Experimental study on small molecule combinations inducing reprogramming of rat fibroblasts into functional neurons.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024Article
- Review
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- Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents.Scientific reports · 2021Article
- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- Adding a dimension to cell fate.Animal reproduction · 2020Article
- All roads lead to Rome: the many ways to pluripotency.Journal of assisted reproduction and genetics · 2020Review
- Conversion of human urine-derived cells into neuron-like cells by small molecules.Molecular biology reports · 2020Article
- Endodermal differentiation of human induced pluripotent stem cells using simple dialysis culture system in suspension culture.Regenerative therapy · 2019Article
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Epigenetic Erasing and Pancreatic Differentiation of Dermal Fibroblasts into Insulin-Producing Cells are Boosted by the Use of Low-Stiffness Substrate.Stem cell reviews and reports · 2018Article
- Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line.PloS one · 2018Article
- Small molecules for reprogramming and transdifferentiation.Cellular and molecular life sciences : CMLS · 2017Review
- 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts.Scientific reports · 2016Article
- Erase and Rewind: Epigenetic Conversion of Cell Fate.Stem cell reviews and reports · 2016Review
- Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts.Journal of visualized experiments : JoVE · 2016Article
- The quest for an effective and safe personalized cell therapy using epigenetic tools.Clinical epigenetics · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large animal models provide useful data for pre-clinical research including regenerative medicine. However whereas the derivation of tissue specific stem cells has been successful. pluripotent stem cells so far have been difficult to obtain in these species. A possible alternative could be direct reprogramming but this has only been described in mouse and human. We have recently described an alternative method for reprogramming human somatic cells based on a brief demethylation step immediately followed by an induction protocol. Aim of the present paper was to determine whether this method is applicable to pig in the attempt to achieve cell reprogramming in a large animal model for the first time. Pig dermal fibroblasts were exposed to DNA methyltransferase inhibitor 5-aza-cytidine (5-aza-CR) for 18 h. After a brief recovery period, fibroblast were subjected to a three-step protocol for the induction of endocrine pancreatic differentiation that was completed after 42 days. During the process pig fibroblast rapidly lost their typical elongated form and gradually became organized in a reticular pattern that evolved into distinct cell aggregates. After a brief expression of some pluripotency genes, cells expression pattern mimicked the transition from primitive endoderm to endocrine pancreas. Not only converted cells expressed insulin but were able to release it in response to a physiological glucose challenge in vitro. Finally they were able to protect recipient mice against streptozotocin-induced diabetes. This work shows, that the conversion of a somatic cell into another, even if belonging to a different germ layer, is possible also in pig.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.