ArticleJournal of dental research2013
M180 amelogenin processed by MMP20 is sufficient for decussating murine enamel.
Article in Journal of dental research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Integrative multi-omics and bioinformatic analysis ofThe Japanese dental science review · 2026Review
- Neuro-Instructive Hydrogels - A Key to Sensory Innervation in Oral Tissues: A Comprehensive Review.International journal of nanomedicine · 2026Review
- Tissue Regeneration on Rise: Dental Hard Tissue Regeneration and Challenges-A Narrative Review.Scientifica · 2024Review
- Cell-scaffold interactions in tissue engineering for oral and craniofacial reconstruction.Bioactive materials · 2023Article
- Mechanisms of Enamel Mineralization Guided by Amelogenin Nanoribbons.Journal of dental research · 2021Review
- MMP20-generated amelogenin cleavage products prevent formation of fan-shaped enamel malformations.Scientific reports · 2021Article
- Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update.Stem cells international · 2020Review
- Enamel biomimetics-fiction or future of dentistry.International journal of oral science · 2019Review
- Dose-Dependent Rescue of KO Amelogenin Enamel by TransgenesFrontiers in physiology · 2017Article
- Integrative Temporo-Spatial, Mineralogic, Spectroscopic, and Proteomic Analysis of Postnatal Enamel Development in Teeth with Limited Growth.Frontiers in physiology · 2017Article
- Tooth enamel protein amelogenin binds to ameloblast cell membrane-mimicking vesicles via its N-terminus.Biochemical and biophysical research communications · 2015Article
- Analysis of enamel development using murine model systems: approaches and limitations.Frontiers in physiology · 2014Review
- Mouse genetic background influences the dental phenotype.Cells, tissues, organs · 2013Article
- Truncated amelogenin and LRAP transgenes improve Amelx null mouse enamel.Matrix biology : journal of the International Society for Matrix BiologyArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Amelogenin (AMELX) and matrix metalloproteinase-20 (MMP20) are essential for proper enamel development. Amelx and Mmp20 mutations cause amelogenesis imperfecta. MMP20, a protease secreted by ameloblasts, is responsible for processing enamel proteins, including AMELX, during the secretory stage of enamel formation. Of at least 16 different amelogenin splice products, the most abundant isoform found in murine ameloblasts and developing enamel is the M180 protein. To understand the role of MMP20 processing of M180 AMELX, we generated AmelxKO/Mmp20KO (DKO) mice with an amelogenin (M180Tg) transgene. We analyzed the enamel phenotype by SEM to determine enamel structure and thickness, µCT, and by nanoindentation to quantify enamel mechanical properties. M180Tg/DKO mouse enamel had 37% of the hardness of M180Tg/AmelxKO teeth and demonstrated a complete lack of normal prismatic architecture. Although molar enamel of M180Tg/AmelxKO mice was thinner than WT, it had similar mechanical properties and decussating enamel prisms, which were abolished by the loss of MMP20 in the M180Tg/DKO mice. Retention of the C-terminus or complete lack of this domain is unable to rescue amelogenin null enamel. We conclude that among amelogenins, M180 alone is sufficient for normal enamel mechanical properties and prism patterns, but that additional amelogenin splice products are required to restore enamel thickness.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.