ArticleNature protocols2013
Upconversion luminescence imaging of cells and small animals.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Synthesis of Upconversion Nanoparticles with Manipulable Crystal Phase, Size Specification, and Morphological Features.Journal of fluorescence · 2025Article
- Noninvasive cardiac modulation via triplet-sensitized photoswitching in the phototherapeutic window.Nature communications · 2025Article
- Water-insensitive NIR-I-to-NIR-I down-shifting nanoparticles enable stable biomarker detection at low power thresholds in opaque aqueous environments.Light, science & applications · 2025Article
- Nanometer-resolution tracking of single cargo reveals dynein motor mechanisms.Nature chemical biology · 2025Article
- Super-photostable organic dye for long-term live-cell single-protein imaging.Nature methods · 2025Article
- Dye-sensitized upconversion nanoprobes with ultra-high signal-to-background ratio for visual and sensitive detection of nerve agent mimics.Mikrochimica acta · 2025Article
- Silica-Based Materials Containing Inorganic Red/NIR Emitters and Their Application in Biomedicine.Materials (Basel, Switzerland) · 2023Review
- Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging.Nature protocols · 2022Review
- Controllable Engineering and Functionalizing of Nanoparticles for Targeting Specific Proteins towards Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Hot-Band-Absorption-Induced Anti-Stokes Fluorescence of Aggregation-Induced Emission Dots and the Influence on the Nonlinear Optical Effect.Biosensors · 2021Article
- Hot-band absorption of indocyanine green for advanced anti-stokes fluorescence bioimaging.Light, science & applications · 2021Article
- Brilliant Nonlinear Optical Response of HoACS omega · 2021Article
- Enhancing Combined Immunotherapy and Radiotherapy through Nanomedicine.Bioconjugate chemistry · 2020Review
- The Renal Clearable Magnetic Resonance Imaging Contrast Agents: State of the Art and Recent Advances.Molecules (Basel, Switzerland) · 2020Review
- Lanthanide-doped mesoporous MCM-41 nanoparticles as a novel optical-magnetic multifunctional nanobioprobe.RSC advances · 2019Article
- Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells.Biomolecules · 2019Article
- NIR-Light-Driven Generation of Reactive Oxygen Species Using Ru(II)-Decorated Lipid-Encapsulated Upconverting Nanoparticles.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy.Nature nanotechnology · 2019Article
- Article
- Over 1000-fold enhancement of upconversion luminescence using water-dispersible metal-insulator-metal nanostructures.Nature communications · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upconversion luminescence (UCL) is an anti-Stokes process whereby low-energy photons are converted to higher-energy ones. UCL imaging for cells and animal tissues has attracted substantial attention in recent years because of the unique abilities of upconversion materials, which can minimize the background interference from the autofluorescence of biosamples and enhance tissue penetration. This protocol describes a step-by-step guide for the fabrication of UCL probes, including lanthanide-based upconversion nanoparticles (Ln-UCNPs) with a particle size of ∼20 nm (NaYF4/NaLuF4: Yb, Er/Tm) and triplet-triplet annihilation-based UCNPs (TTA-UCNPs) with a particle size of ∼10 nm (palladium octaethylporphyrin as sensitizer and 9,10-diphenylanthracene as annihilator). We also describe the characterization of the UCL nanoprobes (via transmission electron microscopy and UCL emission spectroscopy) and functionalization (via silica coating and ligand exchange), as well as applications for UCL bioimaging of living cells (HeLa cells) and small animals (nude mice and Kunming mice). The setup of a laser-scanning UCL microscope and a UCL imaging system is also presented. Compared with a normal imaging setup, we adopted longer-wavelength excitation lasers and short-pass filters. The synthesis of hydrophilic UCNP for application in UCL bioimaging requires ∼15 d.
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