ArticleAnalytical chemistry2013
Isolation and purification of glycoconjugates from complex biological sources by recycling high-performance liquid chromatography.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- [Applications of chromatography in glycomics].Se pu = Chinese journal of chromatography · 2024Review
- Characterization and Analysis of Food-Sourced Carbohydrates.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Preparative scale purification of natural glycans by closed-loop recycle HPLC.Analytical biochemistry · 2020Article
- Preparation of Complex Glycans From Natural Sources for Functional Study.Frontiers in chemistry · 2020Review
- Automated Chemical Oligosaccharide Synthesis: Novel Approach to Traditional Challenges.Chemical reviews · 2018Review
- Isolation of High Purity Anthocyanin Monomers from Red Cabbage with Recycling Preparative Liquid Chromatography and Their Photostability.Molecules (Basel, Switzerland) · 2018Article
- Recent Liquid Chromatographic Approaches and Developments for the Separation and Purification of Carbohydrates.Analytical methods : advancing methods and applications · 2017Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- Protocol for the purification of protected carbohydrates: toward coupling automated synthesis to alternate-pump recycling high-performance liquid chromatography.Chemical communications (Cambridge, England) · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Among of the most urgent needs of the glycobiology community is to generate libraries of pure carbohydrate standards. While many oligosaccharides have recently been synthesized, some glycans of biomedical importance are still missing in existing collections or are available in only limited amounts. To address this need, we demonstrate the use of the relatively unexplored technique of recycling high-performance liquid chromatography (R-HPLC) to isolate and purify glycoconjugates from several natural sources. We were able to routinely achieve purities greater than 98%. In several cases, we were able to obtain isomerically pure substances, particularly for glycans with different positional isomerism. These purified substances can then be used in different analytical applications, for example, as standards for mass spectrometry (MS) and capillary-based separations. Moreover, using a bifunctional aromatic amine, the same derivatization agent can be used to enable UV detection of oligosaccharides during their purification and link the isolated molecules to functionalized surfaces and potentially create glycan arrays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.