ArticleBMC microbiology2013
A structural comparison of lipopolysaccharide biosynthesis loci of Legionella pneumophila serogroup 1 strains.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Combination of antibody-based screening methods for the identification and quantification of Legionella pneumophila in process water.Analytical and bioanalytical chemistry · 2026Article
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- LegioTyper: Rapid typing of Legionella pneumophila serogroup 1 by flow-based chemiluminescence sandwich microarray immunoassay.Analytical and bioanalytical chemistry · 2025Article
- The discovery of aScience advances · 2025Article
- Host-Specific Adaptation of Legionella pneumophila to Single and Multiple Hosts.Molecular biology and evolution · 2025Article
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- Lipopolysaccharide ofInternational journal of molecular sciences · 2023Article
- Presence of Viable, Clinically RelevantMicrobiology spectrum · 2022Article
- Population analysis of Legionella pneumophila reveals a basis for resistance to complement-mediated killing.Nature communications · 2021Article
- Development of a Multiplex-PCR Serotyping Assay for Characterizing Legionella pneumophila Serogroups Based on the Diversity of Lipopolysaccharide Biosynthetic Loci.Journal of clinical microbiology · 2021Article
- The Role of Lipids inInternational journal of molecular sciences · 2021Review
- Distribution ofFrontiers in cellular and infection microbiology · 2019Article
- Genomic heterogeneity differentiates clinical and environmental subgroups of Legionella pneumophila sequence type 1.PloS one · 2018Article
- The Role of Carbohydrates in the Lipopolysaccharide (LPS)/Toll-Like Receptor 4 (TLR4) Signalling.International journal of molecular sciences · 2017Review
- Dynamics and impact of homologous recombination on the evolution of Legionella pneumophila.PLoS genetics · 2017Article
- Complete Genome Sequences of the Historical Legionella pneumophila Strains OLDA and Pontiac.Genome announcements · 2016Article
- Legionella pneumophila strain associated with the first evidence of person-to-person transmission of Legionnaires' disease: a unique mosaic genetic backbone.Scientific reports · 2016Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe lipopolysaccharide (LPS) is the major immuno-dominant antigen of all Legionella species including L. pneumophila. Its diversity is the basis for the classification of L. pneumophila into serogroups and monoclonal subgroups and is thought to be involved in strain specific virulence. The understanding of the genetic basis of the LPS-antigen is incomplete. Thus, we analyzed the genetic locus involved in LPS-biosynthesis of L. pneumophila serogroup 1 (Sg1) strains with the focus on strain specific gene composition.
resultsThe LPS-biosynthesis loci of 14 L. pneumophila Sg1 strains comprise two distinct regions: A 15 kb region containing LPS-biosynthesis genes that can be found in all L. pneumophila strains and a Sg1-specific 18 kb region. The 15 kb region is highly conserved among Sg1 strains as reflected by high homologies of single ORFs and by a consistent ORF arrangement. In contrast, the Sg1 specific 18 kb region is variable and partially disrupted by phage related genes. We propose that the region spanning from ORF 6 to ORF 11 of the Sg1-specific region is likely involved in late LPS-modification. Due to the high variability of this small region and various combinations of single ORFs within this region a strain specific LPS-structure could be synthesized including modifications of legionaminic acid derivates.
conclusionsOur data clearly demonstrate that the gene structure of the LPS-biosynthesis locus of L. pneumophila Sg1 strains show significant interstrain variability. These data can be used for further functional analysis of the LPS synthesis to understand pathogenesis and reactivity with monoclonal antibodies. Moreover, variable but strain specific regions can serve as basis for the development of novel genotyping assays.
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