ArticleBioMed research international2013
Activation of the ERK1/2 signaling pathway during the osteogenic differentiation of mesenchymal stem cells cultured on substrates modified with various chemical groups.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Aging Reprograms the Signaling, Metabolic, and Gene Regulatory Dynamics in Murine Bone Marrow-Derived Mesenchymal Stem Cells.Applied sciences (Basel, Switzerland) · 2026Article
- PTEN Inhibition Suppresses Differentiation in Periodontal Ligament Stem Cells.International journal of molecular sciences · 2026Article
- HDAC inhibitors restore osteoimmune balance and bone regeneration via selective MAPK modulation in inflammatory bone disease.Stem cell research & therapy · 2025Article
- Facile construction of mechanically robust and highly osteogenic materials for bone regeneration.Materials today. Bio · 2025Article
- Advances in the application and research of biomaterials in promoting bone repair and regeneration through immune modulation.Materials today. Bio · 2025Review
- Application and progress of 3D printed biomaterials in osteoporosis.Frontiers in bioengineering and biotechnology · 2025Review
- PI3K/AKT signaling mediate collagen type 1-induced osteogenic differentiation of dental pulp stem cells via focal adhesion mechanism.Journal of applied oral science : revista FOB · 2025Article
- Cell-Tissue Interaction: The Biomimetic Approach to Design Tissue Engineered Biomaterials.Bioengineering (Basel, Switzerland) · 2023Review
- Bladder Cancer Cells Exert Pleiotropic Effects on Human Adipose-Derived Stem Cells.Life (Basel, Switzerland) · 2022Article
- Collagen Modulates the Biological Characteristics of WJ-MSCs in Basal and Osteoinduced Conditions.Stem cells international · 2022Article
- Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling.Frontiers in bioengineering and biotechnology · 2021Review
- Mechanotransduction and Stiffness-Sensing: Mechanisms and Opportunities to Control Multiple Molecular Aspects of Cell Phenotype as a Design Cornerstone of Cell-Instructive Biomaterials for Articular Cartilage Repair.International journal of molecular sciences · 2020Review
- Specifications for Innovative, Enabling Biomaterials Based on the Principles of Biocompatibility Mechanisms.Frontiers in bioengineering and biotechnology · 2019Article
- MAPK signaling has stage-dependent osteogenic effects on human adipose-derived stem cells in vitro.Connective tissue research · 2018Article
- Directing the osteoblastic and chondrocytic differentiations of mesenchymal stem cells: matrix vs. induction media.Regenerative biomaterials · 2017Article
- Review
- Role of the ERK1/2 Signaling Pathway in Osteogenesis of Rat Tendon-Derived Stem Cells in Normoxic and Hypoxic Cultures.International journal of medical sciences · 2016Article
- Organizational metrics of interchromatin speckle factor domains: integrative classifier for stem cell adhesion & lineage signaling.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Innate Immunity and Biomaterials at the Nexus: Friends or Foes.BioMed research international · 2015Review
- Directing the fate of human and mouse mesenchymal stem cells by hydroxyl-methyl mixed self-assembled monolayers with varying wettability.Journal of materials chemistry. B · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current study examined the influence of culture substrates modified with the functional groups -OH, -COOH, -NH2, and -CH3 using SAMs technology, in conjunction with TAAB control, on the osteogenic differentiation of rabbit BMSCs. The CCK-8 assay revealed that BMSCs exhibited substrate-dependent cell viability. The cells plated on -NH2- and -OH-modified substrates were well spread and homogeneous, but those on the -COOH- and -CH3-modified substrates showed more rounded phenotype. The mRNA expression of BMSCs revealed that -NH2-modified substrate promoted the mRNA expression and osteogenic differentiation of the BMSCs. The contribution of ERK1/2 signaling pathway to the osteogenic differentiation of BMSCs cultured on the -NH2-modified substrate was investigated in vitro. The -NH2-modified substrate promoted the expression of integrins; the activation of FAK and ERK1/2. Inhibition of ERK1/2 activation by PD98059, a specific inhibitor of the ERK signaling pathway, blocked ERK1/2 activation in a dose-dependent manner, as revealed for expression of Cbf α -1 and ALP. Blockade of ERK1/2 phosphorylation in BMSCs by PD98059 suppressed osteogenic differentiation on chemical surfaces. These findings indicate a potential role for ERK in the osteogenic differentiation of BMSCs on surfaces modified by specific chemical functional groups, indicating that the microenvironment affects the differentiation of BMSCs. This observation has important implications for bone tissue engineering.
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