ArticlePloS one2013
Solution-phase vs surface-phase aptamer-protein affinity from a label-free kinetic biosensor.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 62 citations in OpenAlex.
- Real-Time Monitoring of Trastuzumab Monoclonal Antibodies Using Electrochemical Aptamer-Based Sensors.ACS sensors · 2026Article
- A smartphone aptasensor for fipronil detection in honey samples.Analytical and bioanalytical chemistry · 2024Article
- Pyoverdine binding aptamers and label-free electrochemical detection of pseudomonads.Frontiers in chemistry · 2024Article
- Comparative Analysis of pH and Target-Induced Conformational Changes of an Oxytetracycline Aptamer in Solution Phase and Surface-Immobilized Form.Biomolecules · 2023Article
- Article
- Structurally Different Yet Functionally Similar: Aptamers Specific for the Ebola Virus Soluble Glycoprotein and GP1,2 and Their Application in Electrochemical Sensing.International journal of molecular sciences · 2023Article
- A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay.Sensors (Basel, Switzerland) · 2021Article
- Sensing with Nanopores and Aptamers: A Way Forward.Sensors (Basel, Switzerland) · 2020Review
- Kinetic Exclusion Assay of Biomolecules by Aptamer Capture.Sensors (Basel, Switzerland) · 2020Article
- Cyclic Peptide Mimetic of Damaged Collagen.Biomacromolecules · 2020Article
- Label free thrombin detection in presence of high concentration of albumin using an aptamer-functionalized nanoporous membrane.Biosensors & bioelectronics · 2019Article
- Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates.ACS applied materials & interfaces · 2018Article
- Aptamer Microarrays-Current Status and Future Prospects.Microarrays (Basel, Switzerland) · 2015Review
- Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays (Small Molecules Detection from Split-Aptamers).Microarrays (Basel, Switzerland) · 2015Article
- Article
- High-throughput binding characterization of RNA aptamer selections using a microplate-based multiplex microcolumn device.Analytical and bioanalytical chemistry · 2014Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aptamers are selected DNA ligands that target biomolecules such as proteins. In recent years, they are showing an increasing interest as potential therapeutic agents or recognition elements in biosensor applications. In both cases, the need for characterizing the mating between the target and the aptamer either in solution or immobilized on a surface, is pressing. In this context, we have developed a kinetic biosensor made of micro-arrayed anti-thrombin aptamers to assess the kinetic parameters of this interaction. The binding of label-free thrombin on the biosensor was monitored in real-time by Surface Plasmon Resonance imaging. Remarkable performances were obtained for the quantification of thrombin without amplification (sub-nanomolar limit of detection and linear range of quantification to two orders of magnitude). The independent determinations of both the solution- and surface-phase affinities, respectively KD(Sol) and KD(Surf), revealed distinct values illustrating the importance of probes, targets or surface interactions in biosensors. Interestingly, KD(Surf) values depend on the aptamer grafting density and linearly extrapolate towards KD(Sol) for highly diluted probes. This suggests a lesser impact of the surface compared to the probe or target cooperativity interactions since the latter decrease with a reduced grafting density.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.