Evidence map›Paper›PMID 24069349›Full record

ArticlePloS one2013

Surface glycosylation profiles of urine extracellular vesicles.

Jared Q Gerlach, Anja Krüger, Susan Gallogly, Shirley A Hanley, Marie C Hogan, Christopher J Ward, Lokesh Joshi, Matthew D Griffin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 109 citations in OpenAlex.

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  16. Principles and Problems of Exosome Isolation from Biological Fluids.Biochemistry (Moscow) Supplement. Series A, Membrane and cell biology · 2022
    Article
  17. Analysis ofPathogens (Basel, Switzerland) · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Jared Q GerlachRegenerative Medicine Institute (REMEDI), National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland ; Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
Anja Krüger
Susan Gallogly
Shirley A Hanley
Marie C Hogan
Christopher J Ward
Lokesh Joshi
Matthew D Griffin
Ollscoil na Gaillimhe – University of Galway · IEMayo Clinic · US

Funding

Analysis of the proteome of PKD-ELVs in polycystic kidney disease and controlsRC1DK086161 · NIDDK · MAYO CLINIC ROCHESTER · PI HOGAN, MARIE C, WARD, CHRISTOPHER J · 2009 to 2010
$946k
NIDDK NIH HHS 5RC1DK086161-02NIDDK NIH HHS RC1 DK086161
6 · The paper itself

Abstract

Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomolecules from their cells of origin. Although uEV-associated proteins and RNA have been studied in detail, little information exists regarding uEV glycosylation characteristics. Surface glycosylation profiling by flow cytometry and lectin microarray was applied to uEVs enriched from urine of healthy adults by ultracentrifugation and centrifugal filtration. The carbohydrate specificity of lectin microarray profiles was confirmed by competitive sugar inhibition and carbohydrate-specific enzyme hydrolysis. Glycosylation profiles of uEVs and purified Tamm Horsfall protein were compared. In both flow cytometry and lectin microarray assays, uEVs demonstrated surface binding, at low to moderate intensities, of a broad range of lectins whether prepared by ultracentrifugation or centrifugal filtration. In general, ultracentrifugation-prepared uEVs demonstrated higher lectin binding intensities than centrifugal filtration-prepared uEVs consistent with lesser amounts of co-purified non-vesicular proteins. The surface glycosylation profiles of uEVs showed little inter-individual variation and were distinct from those of Tamm Horsfall protein, which bound a limited number of lectins. In a pilot study, lectin microarray was used to compare uEVs from individuals with autosomal dominant polycystic kidney disease to those of age-matched controls. The lectin microarray profiles of polycystic kidney disease and healthy uEVs showed differences in binding intensity of 6/43 lectins. Our results reveal a complex surface glycosylation profile of uEVs that is accessible to lectin-based analysis following multiple uEV enrichment techniques, is distinct from co-purified Tamm Horsfall protein and may demonstrate disease-specific modifications.

Indexed as

AdultCarbohydratesCell-Derived MicroparticlesCluster AnalysisExosomesFemaleFlow CytometryGlycosylationHumansLectinsMalePolycystic Kidney, Autosomal DominantProtein Array AnalysisProtein BindingRenal Insufficiency, ChronicUrineCarbohydratesLectinsUromodulin

Identifiers

PMID24069349
PMCPMC3777961
OpenAlexW2055827443

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.