ArticlePLoS genetics2013
Mitochondrial transcription terminator family members mTTF and mTerf5 have opposing roles in coordination of mtDNA synthesis.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 19 citations in OpenAlex.
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- Elucidating Drought Stress Tolerance in European Oaks Through Cross-Species Transcriptomics.G3 (Bethesda, Md.) · 2019Article
- Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo.PLoS genetics · 2019Article
- The mitochondrial R-loop.Nucleic acids research · 2019Article
- Long-read sequencing reveals a 4.4 kb tandem repeat region in the mitogenome of Echinococcus granulosus (sensu stricto) genotype G1.Parasites & vectors · 2019Article
- RNase H1 promotes replication fork progression through oppositely transcribed regions ofThe Journal of biological chemistry · 2019Article
- Expression of the Alternative Oxidase Influences Jun N-Terminal Kinase Signaling and Cell Migration.Molecular and cellular biology · 2018Article
- Structural rearrangements in the mitochondrial genome of Drosophila melanogaster induced by elevated levels of the replicative DNA helicase.Nucleic acids research · 2018Article
- Intracellular vesicle trafficking plays an essential role in mitochondrial quality control.Molecular biology of the cell · 2018Article
- Mitochondrial transcription termination factor 1 directs polar replication fork pausing.Nucleic acids research · 2016Article
- SUV3 helicase is required for correct processing of mitochondrial transcripts.Nucleic acids research · 2015Article
- Article
- Evolution of the metazoan mitochondrial replicase.Genome biology and evolution · 2015Article
- A rolling circle replication mechanism produces multimeric lariats of mitochondrial DNA in Caenorhabditis elegans.PLoS genetics · 2015Article
- Screen for mitochondrial DNA copy number maintenance genes reveals essential role for ATP synthase.Molecular systems biology · 2014Article
- Animal Mitochondrial DNA Replication.The EnzymesReview
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 5 countries.
Funding
Abstract
All genomes require a system for avoidance or handling of collisions between the machineries of DNA replication and transcription. We have investigated the roles in this process of the mTERF (mitochondrial transcription termination factor) family members mTTF and mTerf5 in Drosophila melanogaster. The two mTTF binding sites in Drosophila mtDNA, which also bind mTerf5, were found to coincide with major sites of replication pausing. RNAi-mediated knockdown of either factor resulted in mtDNA depletion and developmental arrest. mTTF knockdown decreased site-specific replication pausing, but led to an increase in replication stalling and fork regression in broad zones around each mTTF binding site. Lagging-strand DNA synthesis was impaired, with extended RNA/DNA hybrid segments seen in replication intermediates. This was accompanied by the accumulation of recombination intermediates and nicked/broken mtDNA species. Conversely, mTerf5 knockdown led to enhanced replication pausing at mTTF binding sites, a decrease in fragile replication intermediates containing single-stranded segments, and the disappearance of species containing segments of RNA/DNA hybrid. These findings indicate an essential and previously undescribed role for proteins of the mTERF family in the integration of transcription and DNA replication, preventing unregulated collisions and facilitating productive interactions between the two machineries that are inferred to be essential for completion of lagging-strand DNA synthesis.
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