ArticleJournal of carbohydrate chemistry2012
Cell-Based Microscale Isolation of Glycoaminoglycans for Glycomics Study.
Article in Journal of carbohydrate chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- High density fermentation of probiotic E. coli Nissle 1917 towards heparosan production, characterization, and modification.Applied microbiology and biotechnology · 2021Article
- A method for characterising human intervertebral disc glycosaminoglycan disaccharides using liquid chromatography-mass spectrometry with multiple reaction monitoring.European cells & materials · 2018Article
- Surprising absence of heparin in the intestinal mucosa of baby pigs.Glycobiology · 2017Article
- Capillary electrophoresis for total glycosaminoglycan analysis.Analytical and bioanalytical chemistry · 2014Article
- CHO microRNA engineering is growing up: recent successes and future challenges.Biotechnology advances · 2013Review
- Microanalysis of stomach cancer glycosaminoglycans.Glycoconjugate journal · 2013Article
- On-line separation and characterization of hyaluronan oligosaccharides derived from radical depolymerization.Carbohydrate polymers · 2013Article
- Microscale separation of heparosan, heparan sulfate, and heparin.Analytical biochemistry · 2013Article
- Recent advances in cellular glycomic analyses.Biomolecules · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, moderately sulfated, and nonsulfated glycosaminoglycans (GAGs) was developed using Chinese hamster ovary (CHO) cells. This microscale isolation relies on a mini-strong anion exchange spin column eluted stepwise with different concentrations of sodium chloride solution. Hyaluronic acid, chondroitin sulfate, and heparin were used to optimize the isolation of the endogenous glycosaminoglycans in CHO cells. This method can also be used to determine the presence of nonsulfated GAGs including heparosan, hyaluronic acid, and nonsulfated chondroitin.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.