ArticleInflammation2014
Binding of CD14 to Mycoplasma genitalium-derived lipid-associated membrane proteins upregulates TNF-α.
Article in Inflammation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Role of P38 lipoprotein in Mycoplasma penetrans adhesion to human urothelial cells.BMC microbiology · 2025Article
- Unraveling the role of distinct cytoskeletal motility structures in Mycoplasma pneumoniae relatives.BMC microbiology · 2025Review
- MreB: unraveling the molecular mechanisms of bacterial shape, division, and environmental adaptation.Cell communication and signaling : CCS · 2025Review
- Advances in adhesion-related pathogenesis inFrontiers in microbiology · 2025Review
- Screening the receptors forFrontiers in cellular and infection microbiology · 2025Article
- Article
- Transcription analysis of the porcine alveolar macrophage response to Mycoplasma hyopneumoniae.PloS one · 2014Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid-associated membrane proteins (LAMPs) are a mixture of mycoplasmal lipoproteins expressed on the surface, and they are the main structures for interaction with the host cells. The objective of this study was to explore the role of CD14 in immune recognition of Mycoplasma genitalium-derived LAMPs and investigate whether the binding of CD14 to LAMPs affects the inflammatory response. Enzyme-linked immunosorbent assay (ELISA), transient co-transfection, dual-luciferase reporter assay, specific inhibition assay, and competitive inhibition ELISA (CI-ELISA) were used. CD14 was involved in LAMP-stimulated production of tumor necrosis factor (TNF)-α by blocking CD14 antibody in THP-1 cells. Co-transfection experiments in HeLa cells provide evidence that CD14 facilitates LAMP-induced TNF-α release via toll-like receptor 2 (TLR2). In addition, LAMP-induced TNF-α release was increased by soluble CD14 but decreased by soluble TLR2. Lipid moieties of LAMPs pre-treated with lipoprotein lipase were responsible for TNF-α production. The binding of CD14 to LAMPs was supported by binding assay and CI-ELISA. Thus, we provide evidences that CD14 is not only able to recognize LAMPs but also its binding to LAMPs upregulates TNF-α release. These findings provide insight into the function of CD14 and the pathogenesis of mycoplasmal infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.