ArticleMolecular biology reports2013
Transcriptome profiling and digital gene expression analysis of Nile tilapia (Oreochromis niloticus) infected by Streptococcus agalactiae.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 82 citations in OpenAlex.
- AFrontiers in immunology · 2026Article
- Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Frontiers in immunology · 2024Article
- Natural infection of hybrid sturgeon (Frontiers in immunology · 2024Article
- Genome-Wide Identification ofMarine drugs · 2023Article
- Applying genetic technologies to combat infectious diseases in aquaculture.Reviews in aquaculture · 2023Review
- Comparative Transcriptomic Immune Responses of Mullet (Frontiers in cellular and infection microbiology · 2022Article
- Comparative Transcriptomic Analysis Reveals the Regulated Expression Profiles inFrontiers in genetics · 2022Article
- Molecular Identification ofFrontiers in immunology · 2022Article
- LECT2 Protects Nile Tilapia (Frontiers in immunology · 2021Article
- Immune-Related Functional Differential Gene Expression in Koi Carp (International journal of molecular sciences · 2018Article
- Characterization of Spleen Transcriptome of Schizothorax prenanti during Aeromonas hydrophila Infection.Marine biotechnology (New York, N.Y.) · 2018Article
- Identifying a Major QTL Associated with Salinity Tolerance in Nile Tilapia Using QTL-Seq.Marine biotechnology (New York, N.Y.) · 2018Article
- Genome-Wide QTL Analysis Identified Significant Associations Between Hypoxia Tolerance and Mutations in the GPR132 and ABCG4 Genes in Nile Tilapia.Marine biotechnology (New York, N.Y.) · 2017Article
- Transcriptomic analysis of the differentiating ovary of the protogynous ricefield eel Monopterus albus.BMC genomics · 2017Article
- De Novo Transcriptome Analysis of Differential Functional Gene Expression in Largemouth Bass (Micropterus salmoides) after Challenge with Nocardia seriolae.International journal of molecular sciences · 2016Article
- De novo characterization of the liver transcriptome of javelin goby Synechogobius hasta and analysis of its transcriptomic profile following waterborne copper exposure.Fish physiology and biochemistry · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tilapia is an important freshwater aquaculture species worldwide. In recent years, streptococcal diseases have severely threatened development of tilapia aquaculture, while effective prevention and control methods have not yet been established. In order to understand the immunological response of tilapia to infection by Streptococcus agalactiae (S. agalactiae), this study employed Solexa/Illumina RNA-seq and digital gene expression (DGE) technology to investigate changes in the tilapia transcriptome before and after S. agalactiae infection. We obtained 82,799 unigenes (mean size: 618 bp) using de novo assembly. Unigenes were annotated by comparing against databases including Nr, Swissprot, cluster of orthologous groups of proteins, Kyoto encyclopedia of genes and genomes, and gene ontology. Combined with DGE technology, transcriptomic changes in tilapia before and after bacteria challenging were examined. A total of 774 significantly up-regulated and 625 significantly down-regulated unigenes were identified, among which 293 were mapped to 181 signaling pathways including 17 immune-related pathways involving 65 differentially expressed genes. We observed a change in the expression of six genes in the Toll-like receptor signaling pathway, and this was subsequently confirmed via quantitative real-time PCR. This comparative study of the tilapia transcriptome before and after S. agalactiae infection identified important differentially-expressed immune-related genes and signaling pathways that will provide useful insights for further analysis of the mechanisms of tilapia defense against S. agalactiae infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.