ArticleJournal of the American Chemical Society2013
DNA catalysts with tyrosine kinase activity.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Probing the Specificity of Fluorescent Deoxyribozymes Using Single-Step Selections and Machine Learning.ACS chemical biology · 2026Article
- The 10-23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions.Angewandte Chemie (International ed. in English) · 2026Review
- DNA Catalysis: Design, Function, and Optimization.Molecules (Basel, Switzerland) · 2024Review
- Supernova: A Deoxyribozyme that Catalyzes a Chemiluminescent Reaction.Angewandte Chemie (International ed. in English) · 2022Article
- Biosensing with DNAzymes.Chemical Society reviews · 2021Review
- Wavelength-Selective Activation of Photocaged DNAzymes for Metal Ion Sensing in Live Cells.ACS omega · 2021Article
- Mimicking Functions of Native Enzymes or Photosynthetic Reaction Centers by Nucleoapzymes and Photonucleoapzymes.Biochemistry · 2021Article
- DNAmoreDB, a database of DNAzymes.Nucleic acids research · 2021Article
- DNAzymes for amine and peptide lysine acylation.Organic & biomolecular chemistry · 2021Article
- Side chain determinants of biopolymer function during selection and replication.Nature chemical biology · 2019Article
- Physical Principles and Extant Biology Reveal Roles for RNA-Containing Membraneless Compartments in Origins of Life Chemistry.Biochemistry · 2018Article
- Nucleobase modification by an RNA enzyme.Nucleic acids research · 2017Article
- Biochemical and Biophysical Understanding of Metal Ion Selectivity of DNAzymes.Inorganica chimica acta · 2016Article
- DNA-catalyzed glycosylation using aryl glycoside donors.Chemical communications (Cambridge, England) · 2016Article
- Catalytic DNA: Scope, Applications, and Biochemistry of Deoxyribozymes.Trends in biochemical sciences · 2016Review
- Assessing histidine tags for recruiting deoxyribozymes to catalyze peptide and protein modification reactions.Organic & biomolecular chemistry · 2016Article
- DNA Oligonucleotide 3'-Phosphorylation by a DNA Enzyme.Biochemistry · 2016Article
- Rational design of supramolecular hemin/G-quadruplex-dopamine aptamer nucleoapzyme systems with superior catalytic performance.Chemical science · 2016Article
- Crystal structure of a DNA catalyst.Nature · 2016Article
- Identification of Sequence-Selective Tyrosine Kinase Deoxyribozymes.Journal of molecular evolution · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We show that DNA catalysts (deoxyribozymes, DNA enzymes) can phosphorylate tyrosine residues of peptides. Using in vitro selection, we identified deoxyribozymes that transfer the γ-phosphoryl group from a 5'-triphosphorylated donor (a pppRNA oligonucleotide or GTP) to the tyrosine hydroxyl acceptor of a tethered hexapeptide. Tyrosine kinase deoxyribozymes that use pppRNA were identified from each of N30, N40, and N50 random-sequence pools. Each deoxyribozyme requires Zn(2+), and most additionally require Mn(2+). The deoxyribozymes have little or no selectivity for the amino acid identities near the tyrosine, but they are highly selective for phosphorylating tyrosine rather than serine. Analogous GTP-dependent DNA catalysts were identified and found to have apparent Km(GTP) as low as ∼20 μM. These findings establish that DNA has the fundamental catalytic ability to phosphorylate the tyrosine side chain of a peptide substrate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.