ArticleOxidative medicine and cellular longevity2013
Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity.
Article in Oxidative medicine and cellular longevity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System ProtectJournal of fungi (Basel, Switzerland) · 2024Article
- Mitochondrial Aconitase and Its Contribution to the Pathogenesis of Neurodegenerative Diseases.International journal of molecular sciences · 2024Review
- Integrative Analysis of Core Genes and Biological Process Involved in Polycystic Ovary Syndrome.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Kinetic and Regulatory Properties ofInternational journal of molecular sciences · 2023Article
- Genetically controlled mtDNA deletions prevent ROS damage by arresting oxidative phosphorylation.eLife · 2022Article
- The metabolic growth limitations of petite cells lacking the mitochondrial genome.Nature metabolism · 2021Article
- Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 Through Phosphorylation of Puf3 inGenetics · 2020Article
- Metabolic Remodeling during Long-Lasting Cultivation of theMicroorganisms · 2020Article
- Organization and dynamics of yeast mitochondrial nucleoids.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- Genetically regulated hepatic transcripts and pathways orchestrate haematological, biochemical and body composition traits.Scientific reports · 2016Article
- A Novel Hybrid Iron Regulation Network Combines Features from Pathogenic and Nonpathogenic Yeasts.mBio · 2016Article
- Cancer-associated isocitrate dehydrogenase mutations induce mitochondrial DNA instability.Human molecular genetics · 2016Article
- Involvement of Iron-Containing Proteins in Genome Integrity in Arabidopsis Thaliana.Genome integrity · 2015Review
- Sudden sensorineural hearing loss and polymorphisms in iron homeostasis genes: new insights from a case-control study.BioMed research international · 2015Article
- The ins and outs of bacterial iron metabolism.Molecular microbiology · 2014Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate. aco1Δ results in mitochondrial DNA (mtDNA) instability. It has been proposed that Aco1 binds to mtDNA and mediates its maintenance. Here we propose an alternative mechanism to account for mtDNA loss in aco1Δ mutant cells. We found that aco1Δ activated the RTG pathway, resulting in increased expression of genes encoding citrate synthase. By deleting RTG1, RTG3, or genes encoding citrate synthase, mtDNA instability was prevented in aco1Δ mutant cells. Increased activity of citrate synthase leads to iron accumulation in the mitochondria. Mutations in MRS3 and MRS4, encoding two mitochondrial iron transporters, also prevented mtDNA loss due to aco1Δ. Mitochondria are the main source of superoxide radicals, which are converted to H2O2 through two superoxide dismutases, Sod1 and Sod2. H2O2 in turn reacts with Fe(2+) to generate very active hydroxyl radicals. We found that loss of Sod1, but not Sod2, prevents mtDNA loss in aco1Δ mutant cells. We propose that mtDNA loss in aco1Δ mutant cells is caused by the activation of the RTG pathway and subsequent iron citrate accumulation and toxicity.
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