ArticlePloS one2013
Proteomic amino-termini profiling reveals targeting information for protein import into complex plastids.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- A snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins.Plant cell reports · 2024Article
- Evaluating possible maternal effect lethality and genetic background effects inbioRxiv : the preprint server for biology · 2024Article
- Evaluating possible maternal effect lethality and genetic background effects in Naa10 knockout mice.PloS one · 2024Article
- In-Depth Characterization of Apoptosis N-Terminome Reveals a Link Between Caspase-3 Cleavage and Posttranslational N-Terminal Acetylation.Molecular & cellular proteomics : MCP · 2023Review
- Localization and characterization θ carbonic anhydrases in Thalassiosira pseudonana.Photosynthesis research · 2023Article
- Molecular structures reveal the origin of spectral variation in cryptophyte light harvesting antenna proteins.Protein science : a publication of the Protein Society · 2023Article
- A bipartite chromatophore transit peptide and N-terminal protein processing in the Paulinella chromatophore.Plant physiology · 2022Article
- Protein Processing in Plant Mitochondria Compared to Yeast and Mammals.Frontiers in plant science · 2022Review
- Using Diatom and Apicomplexan Models to Study the Heme Pathway ofInternational journal of molecular sciences · 2021Article
- The iron-sulfur scaffold protein HCF101 unveils the complexity of organellar evolution in SAR, Haptista and Cryptista.BMC ecology and evolution · 2021Article
- Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation.Molecular systems biology · 2020Article
- Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.The Plant cell · 2020Review
- RSH enzyme diversity for (p)ppGpp metabolism in Phaeodactylum tricornutum and other diatoms.Scientific reports · 2019Article
- New beginnings and new ends: methods for large-scale characterization of protein termini and their use in plant biology.Journal of experimental botany · 2019Review
- Proteomic analysis of the phycobiliprotein antenna of the cryptophyte alga Guillardia theta cultured under different light intensities.Photosynthesis research · 2018Article
- Intracellular metabolic pathway distribution in diatoms and tools for genome-enabled experimental diatom research.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome.eLife · 2017Article
- Responses of the marine diatom Thalassiosira pseudonana to changes in COScientific reports · 2017Article
- The Arabidopsis Chloroplast Stromal N-Terminome: Complexities of Amino-Terminal Protein Maturation and Stability.Plant physiology · 2015Article
- Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
In organisms with complex plastids acquired by secondary endosymbiosis from a photosynthetic eukaryote, the majority of plastid proteins are nuclear-encoded, translated on cytoplasmic ribosomes, and guided across four membranes by a bipartite targeting sequence. In-depth understanding of this vital import process has been impeded by a lack of information about the transit peptide part of this sequence, which mediates transport across the inner three membranes. We determined the mature N-termini of hundreds of proteins from the model diatom Thalassiosira pseudonana, revealing extensive N-terminal modification by acetylation and proteolytic processing in both cytosol and plastid. We identified 63 mature N-termini of nucleus-encoded plastid proteins, deduced their complete transit peptide sequences, determined a consensus motif for their cleavage by the stromal processing peptidase, and found evidence for subsequent processing by a plastid methionine aminopeptidase. The cleavage motif differs from that of higher plants, but is shared with other eukaryotes with complex plastids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.