ArticlePloS one2013
Eicosapentaenoic acid enhances heat stress-impaired intestinal epithelial barrier function in Caco-2 cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- Omega-3 polyunsaturated fatty acids improve intestinal barrier integrity-albeit to a lesser degree than short-chain fatty acids: an exploratory analysis of the randomized controlled LIBRE trial.European journal of nutrition · 2023Trial
- A new type of biomarker for heat stress: insights from immunology.Frontiers in immunology · 2026Review
- Treatment with omega-3-rich fish oil enhances host defense and reduces intestinal colonization by diarrheagenic Escherichia coli.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025Article
- Pretreatment withFrontiers in pharmacology · 2025Article
- Potential of nutraceuticals in celiac disease.Tissue barriers · 2025Review
- Molecular Mechanisms Linking Omega-3 Fatty Acids and the Gut-Brain Axis.Molecules (Basel, Switzerland) · 2024Review
- Fatty Acid-Binding Protein 4-Mediated Regulation Is Pivotally Involved in Retinal Pathophysiology: A Review.International journal of molecular sciences · 2024Review
- Effects of Dietary Supplementations of Vitamin C, Organic Selenium, Betaine, and Pomegranate Peel on Alleviating the Effect of Heat Stress on Growing Rabbits.Animals : an open access journal from MDPI · 2024Article
- Effects of functional nutrients on chicken intestinal epithelial cells induced with oxidative stress.Journal of animal science and technology · 2023Article
- Differential Effects of Oligosaccharides, Antioxidants, Amino Acids and PUFAs on Heat/Hypoxia-Induced Epithelial Injury in a Caco-2/HT-29 Co-Culture Model.International journal of molecular sciences · 2023Article
- Drug-Induced Hyperthermia Review.Cureus · 2022Review
- Role of omega-3 polyunsaturated fatty acids, citrus pectin, and milk-derived exosomes on intestinal barrier integrity and immunity in animals.Journal of animal science and biotechnology · 2022Review
- Novel organic selenium source hydroxy-selenomethionine counteracts the blood-milk barrier disruption and inflammatory response of mice under heat stress.Frontiers in immunology · 2022Article
- The Role of Dietary Fats in the Development and Prevention of Necrotizing Enterocolitis.Nutrients · 2021Review
- Microbiota-gut-brain axis and nutritional strategy under heat stress.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Omega-3 Polyunsaturated Fatty Acids and the Intestinal Epithelium-A Review.Foods (Basel, Switzerland) · 2021Review
- Enhanced intestinal permeability and intestinal co-morbidities in heat strain: A review and case for autodigestion.Temperature (Austin, Tex.) · 2021Review
- Docosahexaenoic acid alleviates cell injury and improves barrier function by suppressing necroptosis signalling in TNF-α-challenged porcine intestinal epithelial cells.Innate immunity · 2020Article
- The Molecular and Mechanistic Insights Based on Gut-Liver Axis: Nutritional Target for Non-Alcoholic Fatty Liver Disease (NAFLD) Improvement.International journal of molecular sciences · 2020Review
- Beyond Heat Stress: Intestinal Integrity Disruption and Mechanism-Based Intervention Strategies.Nutrients · 2020Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveDysfunction of the intestinal epithelial tight junction (TJ) barrier is known to have an important etiologic role in the pathophysiology of heat stroke. N-3 polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), play a role in maintaining and protecting the TJ structure and function. This study is aimed at investigating whether n-3 PUFAs could alleviate heat stress-induced dysfunction of intestinal tight junction.
methodsHuman intestinal epithelial Caco-2 cells were pre-incubated with EPA, DHA or arachidonic acid (AA) and then exposed to heat stress. Transepithelial electrical resistance (TEER) and Horseradish Peroxidase (HRP) permeability were measured to analyze barrier integrity. Levels of TJ proteins, including occludin, ZO-1 and claudin-2, were analyzed by Western blot and localized by immunofluorescence microscopy. Messenger RNA levels were determined by quantitative real time polymerase chain reaction (Q-PCR). TJ morphology was observed by transmission electron microscopy.
resultsEPA effectively attenuated the decrease in TEER and impairment of intestinal permeability in HRP flux induced by heat exposure. EPA significantly elevated the expression of occludin and ZO-1, while DHA was less effective and AA was not at all effective. The distortion and redistribution of TJ proteins, and disruption of morphology were also effectively prevented by pretreatment with EPA.
conclusionThis study indicates for the first time that EPA is more potent than DHA in protecting against heat-induced permeability dysfunction and epithelial barrier damage of tight junction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.