ArticleMolecular biology reports2013
Testosterone promotes vascular endothelial cell migration via upregulation of ROCK-2/moesin cascade.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Guilingji capsules enhances erectile function by promoting testosterone-dependent angiogenesis in the corpus cavernosum.Journal of traditional and complementary medicine · 2025Article
- Genetic determinants of plasma testosterone in male blood donors are associated with altered red blood cell characteristics and survival in cold storage and after transfusion.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2024Article
- NADPH oxidase 4-derived hydrogen peroxide counterbalances testosterone-induced endothelial dysfunction and migration.American journal of physiology. Endocrinology and metabolism · 2024Article
- Sex, hormones and cerebrovascular function: from development to disorder.Fluids and barriers of the CNS · 2024Review
- Serum dihydrotestosterone levels are associated with adverse myocardial remodeling in patients with severe aortic valve stenosis before and after aortic valve replacement.American journal of physiology. Heart and circulatory physiology · 2022Article
- DLC-1 is an independent prognostic marker and potential therapeutic target in hepatocellular cancer.Diagnostic pathology · 2016Article
- Erectile dysfunction.Nature reviews. Disease primers · 2016Review
- Androgens Regulate T47D Cells Motility and Invasion through Actin Cytoskeleton Remodeling.Frontiers in endocrinology · 2016Article
- Androgen Modulates Functions of Endothelial Progenitor Cells through Activated Egr1 Signaling.Stem cells international · 2016Article
- Pathway analysis supports association of nonsyndromic cryptorchidism with genetic loci linked to cytoskeleton-dependent functions.Human reproduction (Oxford, England) · 2015Article
- 2-methoxyestradiol induces vasodilation by stimulating NO release via PPARγ/PI3K/Akt pathway.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cross-sectional studies have demonstrated a reverse relationship between serum level of testosterone (T) and the incidence rate of cardiovascular disease in men, indicating that T exerts beneficial effects in cardiovascular system. However, the endothelial effects of T are poorly understood. Actin remodeling is essential for endothelial cell movement and vascular repair and this process is controlled by the actin-binding protein moesin. In the present study, we studied the effects of T on actin remodeling, moesin expression and phosphorylation, as well as cell migration in cultured human umbilical endothelial cells (hUVECs). We found that T provoked the formation of cortical actin complexes and membrane protrusions in endothelial cells. Treatment with T induced dose- and time-dependent increase of moesin expression and phosphorylation, which was inhibited by the addition of androgen receptor antagonist hydroxyflutamide (HF). Moreover, T enhanced ROCK-2 activity. The ROCK-2 inhibitor Y27632 or the transfection of ROCK-2 siRNA largely inhibited T-induced moesin expression and phosphorylation, indicating that ROCK-2 pathway is crucial for these effects. T promoted endothelial cell migration, which was inhibited by the addition of HF or Y27632. In conclusion, T induces actin cytoskeleton remodeling by regulating moesin expression and activation, resulting in enhanced endothelial cell migration. Our work adds new insights into endothelial mechanisms of T, which is relevant for its vascular actions.
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