ArticleInternational journal of molecular sciences2013
Spectroscopic studies of model photo-receptors: validation of a nanosecond time-resolved micro-spectrophotometer design using photoactive yellow protein and α-phycoerythrocyanin.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein.Nature communications · 2020Article
- Watching Proteins Function with Time-resolved X-ray Crystallography.Journal of physics D: Applied physics · 2017Article
- A short history of structure based research on the photocycle of photoactive yellow protein.Structural dynamics (Melville, N.Y.) · 2017Review
- Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.Science (New York, N.Y.) · 2016Article
- Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.Science (New York, N.Y.) · 2014Article
- Protein energy landscapes determined by five-dimensional crystallography.Acta crystallographica. Section D, Biological crystallography · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time-resolved spectroscopic experiments have been performed with protein in solution and in crystalline form using a newly designed microspectrophotometer. The time-resolution of these experiments can be as good as two nanoseconds (ns), which is the minimal response time of the image intensifier used. With the current setup, the effective time-resolution is about seven ns, determined mainly by the pulse duration of the nanosecond laser. The amount of protein required is small, on the order of 100 nanograms. Bleaching, which is an undesirable effect common to photoreceptor proteins, is minimized by using a millisecond shutter to avoid extensive exposure to the probing light. We investigate two model photoreceptors, photoactive yellow protein (PYP), and α-phycoerythrocyanin (α-PEC), on different time scales and at different temperatures. Relaxation times obtained from kinetic time-series of difference absorption spectra collected from PYP are consistent with previous results. The comparison with these results validates the capability of this spectrophotometer to deliver high quality time-resolved absorption spectra.
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Registered trials
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