ArticleJournal of experimental botany2013
Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it, 71 citations in OpenAlex.
- Overview of molecular mechanisms of plant leaf development: a systematic review.Frontiers in plant science · 2023Pooled it
- Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks.International journal of molecular sciences · 2018Pooled it
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- Gene Editing of Nicotiana benthamiana Architecture for Space-Efficient Production of Recombinant Proteins in Closed Environments.Plant biotechnology journal · 2026Article
- DRMY1 promotes robust morphogenesis in Arabidopsis by sustaining the translation of cytokinin-signaling inhibitor proteins.Developmental cell · 2024Article
- Divergent Molecular Responses to Heavy Water inPlants (Basel, Switzerland) · 2024Article
- Annotation of thePlants (Basel, Switzerland) · 2023Article
- Cytokinin Deficiency Alters Leaf Proteome and Metabolome during Effector-Triggered Immunity inPlants (Basel, Switzerland) · 2022Article
- Nitrogen application and differences in leaf number retained after topping affect the tobacco (Nicotiana tabacum) transcriptome and metabolome.BMC plant biology · 2022Article
- Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Physiological and metabolic bases of increased growth in the tomato ethylene-insensitive mutant Never ripe: extending ethylene signaling functions.Plant cell reports · 2021Article
- Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level.Plant methods · 2021Article
- The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents.Frontiers in plant science · 2021Article
- Proteome-Wide Analysis of Heat-Stress inFrontiers in plant science · 2021Article
- Induction of Radiata Pine Somatic Embryogenesis at High Temperatures Provokes a Long-Term Decrease in DNA Methylation/Hydroxymethylation and Differential Expression of Stress-Related Genes.Plants (Basel, Switzerland) · 2020Article
- Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome.Plants (Basel, Switzerland) · 2020Article
- Silencing ofInternational journal of molecular sciences · 2020Article
- Halotropism: Phytohormonal Aspects and Potential Applications.Frontiers in plant science · 2020Review
- Light Regulates the Cytokinin-Dependent Cold Stress Responses inFrontiers in plant science · 2020Article
- Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified >1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development, and/or hormone and light signalling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates, and organic acids. Comparison of the data sets obtained from activated CaMV35S>GR>HvCKX2 and CaMV35S>GR>ipt plants revealed unexpectedly extensive overlaps. Integration of the proteomic and metabolomic data sets revealed: (i) novel components of molecular circuits involved in CK action (e.g. ribosomal proteins); (ii) previously unrecognized links to redox regulation and stress hormone signalling networks; and (iii) CK content markers. The striking overlaps in profiles observed in CK-deficient and CK-overproducing seedlings might explain surprising previously reported similarities between plants with down- and up-regulated CK levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.