ArticleEndocrinology2013
GATA4 and GATA6 silencing in ovarian granulosa cells affects levels of mRNAs involved in steroidogenesis, extracellular structure organization, IGF-I activity, and apoptosis.
Article in Endocrinology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Platelet-rich plasma and folliculogenesis: a systematic mechanistic review and computational pathway analysis.Frontiers in reproductive health · 2026Pooled it
- Molecular evidence for early deuterostome origins of ovarian cell types and neuroendocrine control of reproduction.Nature communications · 2026Article
- Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation.Science advances · 2026Article
- An ovine model shows that subcutaneous adipose tissue fibrosis occurs early in polycystic ovary syndrome (PCOS).Journal of molecular endocrinology · 2025Article
- Identification of FSH-regulated and estrous stage-specific transcriptional networks in mouse ovaries.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Adamts9 is required for the development of primary ovarian follicles and maintenance of female sex in zebrafish†.Biology of reproduction · 2024Article
- An integrated analysis of multiple datasets reveals novel gene signatures in human granulosa cells.Scientific data · 2024Article
- Human-relevant exposure to di-n-butyl phthalate tampers with the ovarian insulin-like growth factor 1 system and disrupts folliculogenesis in young adult mice.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Insulin-like growth factor 1 enhances follicle-stimulating hormone-induced phosphorylation of GATA4 in rat granulosa cells.Molecular and cellular endocrinology · 2023Article
- Article
- gga-miR-449b-5p Regulates Steroid Hormone Synthesis in Laying Hen Ovarian Granulosa Cells by Targeting the IGF2BP3 Gene.Animals : an open access journal from MDPI · 2022Article
- Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1.Autophagy · 2022Article
- Mechanism of negative modulation of FSH signaling by salt-inducible kinases in rat granulosa cells.Frontiers in endocrinology · 2022Article
- Comparative Transcriptome Analysis Provides Insight into Spatio-Temporal Expression Characteristics and Genetic Regulatory Network in Postnatal Developing Subcutaneous and Visceral Fat of Bama Pig.Frontiers in genetics · 2022Article
- ERβ Regulation of Gonadotropin Responses during Folliculogenesis.International journal of molecular sciences · 2021Review
- Granulosa cell genes that regulate ovarian follicle development beyond the antral stage: The role of estrogen receptor β.Molecular and cellular endocrinology · 2021Article
- Salt-inducible Kinases Are Critical Determinants of Female Fertility.Endocrinology · 2020Article
- Characterization of oogonia stem cells in mice by Fragilis.Protein & cell · 2019Article
- Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization.Journal of assisted reproduction and genetics · 2018Article
- GATA Regulation and Function During the Ovarian Life Cycle.Vitamins and hormones · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Knockdown of the transcription factors GATA4 and GATA6 in granulosa cells (GCs) impairs folliculogenesis and induces infertility. To investigate the pathways and genes regulated by these factors, we performed microarray analyses on wild-type GCs or GCs lacking GATA4, GATA6, or GATA4/6 (G4(gcko), G6(gcko), and G4/6(gcko)) after in vivo treatment with equine chorionic gonadotropin. GATA4 deletion affected a greater number of genes than GATA6, which correlates with the subfertility observed in G4(gcko) mice and the normal reproductive function found in G6(gcko) animals. An even greater number of genes were affected by the deletion of both factors. Moreover, the expression of FSH receptor, LH receptor, inhibin α and β, versican, pregnancy-associated plasma protein A, and the regulatory unit 2b of protein kinase A, which are known to be crucial for ovarian function, was greatly affected in double GATA4 and GATA6 knockouts when compared with single GATA-deficient animals. This suggests that GATA4 and GATA6 functionally compensate for each other in the regulation of key ovarian genes. Functional enrichment revealed that ovulation, growth, intracellular signaling, extracellular structure organization, gonadotropin and growth factor actions, and steroidogenesis were significantly regulated in G4/6(gcko) mice. The results of this analysis were confirmed using quantitative polymerase chain reaction, immunohistochemical, and biological assays. Treatment of GCs with cAMP/IGF-I, to bypass FSH and IGF-I signaling defects, revealed that most of the affected genes are direct targets of GATA4/6. The diversity of pathways affected by the knockdown of GATA underscores the important role of these factors in the regulation of GC function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.