ArticleThe Journal of biological chemistry2013
Aspergillus niger β-glucosidase has a cellulase-like tadpole molecular shape: insights into glycoside hydrolase family 3 (GH3) β-glucosidase structure and function.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 72 citations in OpenAlex.
- Characterization of a Novel GH3 β-Glucosidase with Alkali-Tolerant and Halophilic Properties from Pontixanthobacter luteolus SW-109.Applied biochemistry and biotechnology · 2026Article
- Genome evolution and transposable element expansion reveal host-associated genomic features in Cladosporium cucumerinum.Communications biology · 2026Article
- Whole-Genome Resequencing ofInternational journal of molecular sciences · 2026Article
- Identification of Laccase Family ofInternational journal of molecular sciences · 2024Article
- Deciphering domain structures of Aspergillus and Streptomyces GH3-β-Glucosidases: a screening system for enzyme engineering and biotechnological applications.BMC research notes · 2024Article
- Genome-wide analysis of the Pleurotus eryngii laccase gene (PeLac) family and functional identification of PeLac5.AMB Express · 2023Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- Recombinant Family 1 Carbohydrate-Binding Modules Derived From Fungal Cellulase Enhance Enzymatic Degradation of Lignocellulose as Novel Effective Accessory Protein.Frontiers in microbiology · 2022Article
- Production of a β-Glucosidase-Rich Cocktail fromJournal of fungi (Basel, Switzerland) · 2021Article
- Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function.Animal bioscience · 2021Article
- Effect of Concentrated Salts Solutions on the Stability of Immobilized Enzymes: Influence of Inactivation Conditions and Immobilization Protocol.Molecules (Basel, Switzerland) · 2021Article
- Proteomic analysis of Aspergillus niger 3.316 under heat stress.MicrobiologyOpen · 2020Article
- Carbohydrate Binding Modules: Diversity of Domain Architecture in Amylases and Cellulases From Filamentous Microorganisms.Frontiers in bioengineering and biotechnology · 2020Review
- Presence of carbohydrate binding modules in extracellular region of class C G-protein coupled receptors (C GPCR): AnJournal of biosciences · 2019Article
- Crystal Structure of a GH3 β-Glucosidase from the Thermophilic FungusInternational journal of molecular sciences · 2019Article
- First Insights into the Microbiome of a Mangrove Tree Reveal Significant Differences in Taxonomic and Functional Composition among Plant and Soil Compartments.Microorganisms · 2019Article
- Studies of adsorptive capacity of bacterial β-glucosidases on lignocresol aiming the enzymatic recycling in bioprocesses.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Enzymatic sugar production from elephant grass and reed straw through pretreatments and hydrolysis with addition of thioredoxin-His-S.Biotechnology for biofuels · 2019Article
- Structural studies of a glycoside hydrolase family 3 β-glucosidase from the model fungus Neurospora crassa.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Metagenomics Investigation of Agarlytic Genes and Genomes in Mangrove Sediments in China: A Potential Repertory for Carbohydrate-Active Enzymes.Frontiers in microbiology · 2018Article
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9 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Aspergillus niger is known to secrete large amounts of β-glucosidases, which have a variety of biotechnological and industrial applications. Here, we purified an A. niger β-glucosidase (AnBgl1) and conducted its biochemical and biophysical analyses. Purified enzyme with an apparent molecular mass of 116 kDa forms monomers in solution as judged by native gel electrophoresis and has a pI value of 4.55, as found for most of the fungi of β-glucosidases. Surprisingly, the small angle x-ray experiments reveal that AnBgl1 has a tadpole-like structure, with the N-terminal catalytic domain and C-terminal fibronectin III-like domain (FnIII) connected by the long linker peptide (∼100 amino acid residues) in an extended conformation. This molecular organization resembles the one adopted by other cellulases (such as cellobiohydrolases, for example) that frequently contain a catalytic domain linked to the cellulose-binding module that mediates their binding to insoluble and polymeric cellulose. The reasons why AnBgl1, which acts on the small soluble substrates, has a tadpole molecular shape are not entirely clear. However, our enzyme pulldown assays with different polymeric substrates suggest that AnBgl1 has little or no capacity to bind to and to adsorb cellulose, xylan, and starch, but it has high affinity to lignin. Molecular dynamics simulations suggested that clusters of residues located in the C-terminal FnIII domain interact strongly with lignin fragments. The simulations showed that numerous arginine residues scattered throughout the FnIII surface play an important role in the interaction with lignin by means of cation-π stacking with the lignin aromatic rings. These results indicate that the C-terminal FnIII domain could be operational for immobilization of the enzyme on the cell wall and for the prevention of unproductive binding of cellulase to the biomass lignin.
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