ArticleThe Journal of experimental medicine1990
Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures.
Article in The Journal of experimental medicine, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
106 citing papers in PubMed, 563 citations in OpenAlex.
- Spatial proteomics identifies a spectrum of immune dysregulation in acquired bone marrow failure syndromes.Frontiers in immunology · 2023Article
- Review
- Isolation and characterization of cells derived from human epithelial rests of Malassez.Odontology · 2019Article
- The Receptor for Hyaluronan-Mediated Motility (CD168) promotes inflammation and fibrosis after acute lung injury.Matrix biology : journal of the International Society for Matrix Biology · 2019Article
- The role of CD44 in fetal and adult hematopoietic stem cell regulation.Haematologica · 2016Article
- CD44, Hyaluronan, the Hematopoietic Stem Cell, and Leukemia-Initiating Cells.Frontiers in immunology · 2015Review
- CD44 standard and CD44v10 isoform expression on leukemia cells distinctly influences niche embedding of hematopoietic stem cells.Journal of hematology & oncology · 2014Article
- Article
- Hyaluronan enhances bone marrow cell therapy for myocardial repair after infarction.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- The interaction between CD44 on tumour cells and hyaluronan under physiologic flow conditions: implications for metastasis formation.Histochemistry and cell biology · 2012Article
- The biology of CD44 and HCELL in hematopoiesis: the 'step 2-bypass pathway' and other emerging perspectives.Current opinion in hematology · 2011Review
- CD44: can a cancer-initiating cell profit from an abundantly expressed molecule?Nature reviews. Cancer · 2011Review
- Anti-CD44 induces apoptosis in T lymphoma via mitochondrial depolarization.Journal of cellular and molecular medicine · 2010Article
- Co-culture with mesenchymal stromal cells increases proliferation and maintenance of haematopoietic progenitor cells.Journal of cellular and molecular medicine · 2010Article
- Dlk1 influences differentiation and function of B lymphocytes.Stem cells and development · 2008Article
- Hyaluronan and CD44 antagonize mitogen-dependent cyclin D1 expression in mesenchymal cells.The Journal of cell biology · 2007Article
- Expression and role of the hyaluronan receptor RHAMM in inflammation after bleomycin injury.American journal of respiratory cell and molecular biology · 2005Article
- RHAMM, a receptor for hyaluronan-mediated motility, compensates for CD44 in inflamed CD44-knockout mice: a different interpretation of redundancy.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- CD44 in cancer progression: adhesion, migration and growth regulation.Journal of molecular histology · 2004Review
- Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1).Leukemia · 2003Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A new panel of mAbs was prepared to a stromal cell line known to support lymphocytes in Whitlock-Witte type long-term bone marrow cultures. These antibodies were then screened with a cell adhesion assay and four were selected that inhibited the binding of B lineage cells to stromal cell monolayers. Immunofluorescent and biochemical analyses revealed that these new antibodies detected epitopes of the previously described Pgp-1/CD44 antigen complex. Addition of Pgp-1/CD44 antibodies to Dexter-type long-term bone marrow cultures completely prevented emergence of myeloid cells and they also blocked lymphocyte growth in Whitlock-Witte type cultures. mAbs MEL-14, LFA-1, and CD45R did not inhibit under the same conditions and there was no apparent relationship to Ig isotype. Adherent layers in treated cultures were not unusual in terms of morphology and the antibodies did not affect factor-dependent replication of lymphoid or myeloid progenitor cells. Therefore, the mechanism of inhibition may not involve direct toxicity to precursors or microenvironmental elements. Previous studies in humans and mice have implicated Pgp-1/CD44-related glycoproteins in the migration of peripheral lymphoid cells, as well as interactions of cells with the extracellular matrix. These findings suggest that they may also be critical for formation of lymphoid and myeloid cells within bone marrow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.