ArticleThe Journal of experimental medicine1990
The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments.
Article in The Journal of experimental medicine, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 176 citations in OpenAlex.
- Metacyclogenesis as the Starting Point of Chagas Disease.International journal of molecular sciences · 2023Review
- Down the membrane hole: Ion channels in protozoan parasites.PLoS pathogens · 2022Review
- Drug Repurposing in Chagas Disease: Chloroquine Potentiates Benznidazole Activity against Trypanosoma cruziAntimicrobial agents and chemotherapy · 2022Article
- Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy.Microbiology spectrum · 2022Article
- Article
- Mechanisms Associated withLife (Basel, Switzerland) · 2021Review
- Transcriptional Studies onFrontiers in cellular and infection microbiology · 2021Review
- All Roads Lead to Cytosol:Frontiers in cellular and infection microbiology · 2021Review
- Endocytic Rabs Are Recruited to theFrontiers in cellular and infection microbiology · 2020Article
- The Parasitic Intracellular Lifestyle of Trypanosomatids: Parasitophorous Vacuole Development and Survival.Frontiers in cell and developmental biology · 2020Review
- Cytosolic Fe-superoxide dismutase safeguardsProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Intracellular development ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2018Article
- Article
- The Uptake and Metabolism of Amino Acids, and Their Unique Role in the Biology of Pathogenic Trypanosomatids.Pathogens (Basel, Switzerland) · 2018Review
- Targeting host mitochondria: A role for the Trypanosoma cruzi amastigote flagellum.Cellular microbiology · 2018Article
- The glutamine synthetase of Trypanosoma cruzi is required for its resistance to ammonium accumulation and evasion of the parasitophorous vacuole during host-cell infection.PLoS neglected tropical diseases · 2018Article
- Modulation of host central carbon metabolism and in situ glucose uptake by intracellular Trypanosoma cruzi amastigotes.PLoS pathogens · 2017Article
- Cardiomyocyte oxidants production may signal to T. cruzi intracellular development.PLoS neglected tropical diseases · 2017Article
- Galectin-3: A Friend but Not a Foe duringFrontiers in cellular and infection microbiology · 2017Article
- Trypanosoma cruzi Differentiates and Multiplies within Chimeric Parasitophorous Vacuoles in Macrophages Coinfected with Leishmania amazonensis.Infection and immunity · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The protozoan parasite Trypanosoma cruzi can infect many distinct mammalian cell types. The parasites enter cells through the formation of phagocytic vacuoles, but later are found free in the cytosol, where they multiply as amastigotes. Using transmission electron microscopy we found that within 2 h after infection 70% of the parasites, including examples of both mammalian forms (trypomastigotes and amastigotes), were inside partially disrupted vacuoles or free in the cytosol. We demonstrated that the pH of vacuoles containing recently interiorized parasites is acidic, through immunocytochemical localization of the acidotropic compound DAMP (18) in their interior. Increasing the vacuolar pH with chloroquine, ammonium chloride, methylamine, or monensin significantly inhibited the escape of the parasites into the cytosol. These results are compatible with the hypothesis that an acid-active hemolysin of T. cruzi (15) might be involved in the escape mechanism.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.