ArticleJournal of industrial microbiology & biotechnology2013
Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 78 citations in OpenAlex.
- Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review.Biotechnology letters · 2025Review
- Boosting Geranyl Diphosphate Synthesis for Linalool Production in Engineered Yarrowia lipolytica.Applied biochemistry and biotechnology · 2024Article
- Biosynthesis of Fatty Acid Derivatives by RecombinantJournal of fungi (Basel, Switzerland) · 2023Article
- Biotechnological production of omega-3 fatty acids: current status and future perspectives.Frontiers in microbiology · 2023Review
- The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the YeastInternational journal of molecular sciences · 2021Article
- Review
- Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin.Applied microbiology and biotechnology · 2021Review
- Production of Long Chain Fatty Alcohols Found in Bumblebee Pheromones byFrontiers in bioengineering and biotechnology · 2020Article
- Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica.Microbial cell factories · 2019Review
- A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology.Microbial biotechnology · 2019Article
- Production and characterization of two medium-chain-length polydroxyalkanoates by engineered strains of Yarrowia lipolytica.Microbial cell factories · 2019Article
- Bioreactor-Scale Strategies for the Production of Recombinant Protein in the YeastMicroorganisms · 2019Review
- Utilization of Wheat Bran Acid Hydrolysate byBioMed research international · 2019Article
- Synthetic biology tools for engineering Yarrowia lipolytica.Biotechnology advances · 2018Review
- Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations.Molecular biotechnology · 2018Review
- Engineering Yarrowia lipolytica to Simultaneously Produce Lipase and Single Cell Protein from Agro-industrial Wastes for Feed.Scientific reports · 2018Article
- Efficient metabolic evolution of engineeredBiotechnology for biofuels · 2018Article
- Alternative Substrate Metabolism inFrontiers in microbiology · 2018Review
- Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis.Microbial cell factories · 2017Article
- Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica.Microbial cell factories · 2017Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yarrowia lipolytica requires the expression of a heterologous invertase to grow on a sucrose-based substrate. This work reports the construction of an optimized invertase expression cassette composed of Saccharomyces cerevisiae Suc2p secretion signal sequence followed by the SUC2 sequence and under the control of the strong Y. lipolytica pTEF promoter. This new construction allows a fast and optimal cleavage of sucrose into glucose and fructose and allows cells to reach the maximum growth rate. Contrary to pre-existing constructions, the expression of SUC2 is not sensitive to medium composition in this context. The strain JMY2593, expressing this new cassette with an optimized secretion signal sequence and a strong promoter, produces 4,519 U/l of extracellular invertase in bioreactor experiments compared to 597 U/l in a strain expressing the former invertase construction. The expression of this cassette strongly improved production of invertase and is suitable for simultaneously high production level of citric acid from sucrose-based media.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.