ArticleIntegrative biology : quantitative biosciences from nano to macro2013
A co-culture device with a tunable stiffness to understand combinatorial cell-cell and cell-matrix interactions.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A pumpless liver-adipose model for studying metabolic dysfunction and drug responses.PloS one · 2026Article
- Biomechanical research using advanced micro-nano devices: In-Vitro cell Characterization focus.Journal of advanced research · 2025Review
- Light-Controlled Cell-Cell Assembly Using Photocaged Oligonucleotides.ACS materials Au · 2023Article
- A Study on the Effect of Doping Metallic Nanoparticles on Fracture Properties of Polylactic Acid Nanofibres via Molecular Dynamics Simulation.Nanomaterials (Basel, Switzerland) · 2023Article
- Simple, Affordable, and Modular Patterning of Cells using DNA.Journal of visualized experiments : JoVE · 2021Article
- Fibroblast growth factor-4 maintains cellular viability while enhancing osteogenic differentiation of stem cell spheroids in part by regulating RUNX2 and BGLAP expression.Experimental and therapeutic medicine · 2020Article
- Anatase Incorporation to Bioactive Scaffolds Based on Salmon Gelatin and Its Effects on Muscle Cell Growth.Polymers · 2020Article
- Hierarchical shape-by-shape assembly of microparticles for micrometer-scale viral delivery of two different genes.Biomicrofluidics · 2018Article
- Hybrid Protein-Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation.ACS omega · 2017Article
- Effect of matrix stiffness on osteoblast functionalization.Cell proliferation · 2017Article
- Patterning of sharp cellular interfaces with a reconfigurable elastic substrate.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds.Journal of materials science. Materials in medicine · 2016Article
- Development of an infusion bioreactor for the accelerated preparation of decellularized skeletal muscle scaffolds.Biotechnology progress · 2016Article
- Synthetic hydrogels with stiffness gradients for durotaxis study and tissue engineering scaffolds.Tissue engineering and regenerative medicine · 2016Review
- Mechanical characterization of electrospun gelatin scaffolds cross-linked by glucose.Journal of materials science. Materials in medicine · 2015Article
Corrections and comments
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Authors and funding
9 authors.
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Abstract
Cell behavior on 2-D in vitro cultures is continually being improved to better mimic in vivo physiological conditions by combining niche cues including multiple cell types and substrate stiffness, which are well known to impact cell phenotype. However, no system exists in which a user can systematically examine cell behavior on a substrate with a specific stiffness (elastic modulus) in culture with a different cell type, while maintaining distinct cell populations. We demonstrate the modification of a silicon reconfigurable co-culture system with a covalently linked hydrogel of user-defined stiffness. This device allows the user to control whether two separate cell populations are in contact with each other or only experience paracrine interactions on substrates of controllable stiffness. To illustrate the utility of this device, we examined the role of substrate stiffness combined with myoblast co-culture on adipose derived stem cell (ASC) differentiation and found that the presence of myoblasts and a 10 kPa substrate stiffness increased ASC myogenesis versus co-culture on stiff substrates. As this example highlights, this technology better controls the in vitro microenvironment, allowing the user to develop a more thorough understanding of the combined effects of cell-cell and cell-matrix interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.