ArticleAnalytical chemistry2013
Development of a capillary electrophoresis platform for identifying inhibitors of protein-protein interactions.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Microfluidics for protein interaction studies: current methods, challenges, and future perspectives.European biophysics journal : EBJ · 2026Review
- Fragment-based drug discovery for transthyretin kinetic stabilisers using a novel capillary zone electrophoresis method.PloS one · 2025Article
- Label-free methods for opticalPhysical chemistry chemical physics : PCCP · 2021Review
- Microfluidic approaches for the analysis of protein-protein interactions in solution.Biophysical reviews · 2020Review
- Droplet sample introduction to microchip gel and zone electrophoresis for rapid analysis of protein-protein complexes and enzymatic reactions.Analytical and bioanalytical chemistry · 2019Article
- Exploration of Benzothiazole Rhodacyanines as Allosteric Inhibitors of Protein-Protein Interactions with Heat Shock Protein 70 (Hsp70).Journal of medicinal chemistry · 2018Article
- Protein-Protein Interactions in the Molecular Chaperone Network.Accounts of chemical research · 2018Review
- Protein cross-linking capillary electrophoresis at increased throughput for a range of protein-protein interactions.The Analyst · 2018Article
- Advances in capillary electrophoresis and the implications for drug discovery.Expert opinion on drug discovery · 2017Review
- Stabilizing the Hsp70-Tau Complex Promotes Turnover in Models of Tauopathy.Cell chemical biology · 2016Article
- Protein Cross-Linking Capillary Electrophoresis for Protein-Protein Interaction Analysis.Analytical chemistry · 2016Article
- Microfluidics: reframing biological enquiry.Nature reviews. Molecular cell biology · 2015Review
- Direct and Propagated Effects of Small Molecules on Protein-Protein Interaction Networks.Frontiers in bioengineering and biotechnology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Methods for identifying chemical inhibitors of protein-protein interactions (PPIs) are often prone to discovery of false positives, particularly those caused by molecules that induce protein aggregation. Thus, there is interest in developing new platforms that might allow earlier identification of these problematic compounds. Capillary electrophoresis (CE) has been evaluated as a method to screen for PPI inhibitors using the challenging system of Hsp70 interacting with its co-chaperone Bag3. In the method, Hsp70 is labeled with a fluorophore, mixed with Bag3, and the resulting bound and free Hsp70 are separated and detected by CE with laser-induced fluorescence detection. The method used a chemically modified CE capillary to prevent protein adsorption. Inhibitors of the Hsp70-Bag3 interaction were detected by observing a reduction in the bound-to-free ratio. The method was used to screen a library of 3443 compounds, and the results were compared to those from a flow cytometry protein interaction assay. CE was found to produce a lower hit rate with more compounds that were reconfirmed in subsequent testing, suggesting greater specificity. This finding was attributed to the use of electropherograms to detect artifacts such as aggregators and to differences in protein modifications required to perform the different assays. Increases in throughput are required to make the CE method suitable for primary screens, but at the current stage of development it is attractive as a secondary screen to test hits found by higher-throughput methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.