ReviewBiochemical Society transactions2013
Rational design of FRET sensor proteins based on mutually exclusive domain interactions.
Review in Biochemical Society transactions, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Bioluminescence Color-Tuning Firefly Luciferases: Engineering and Prospects for Real-Time Intracellular pH Imaging and Heavy Metal Biosensing.Biosensors · 2022Review
- Metal-Dependent DNAzymes for the Quantitative Detection of Metal Ions in Living Cells: Recent Progress, Current Challenges, and Latest Results on FRET Ratiometric Sensors.Inorganic chemistry · 2019Review
- Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.BioMed research international · 2019Review
- Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species.Molecules (Basel, Switzerland) · 2018Article
- Intestinal Farnesoid X Receptor Activation by Pharmacologic Inhibition of the Organic Solute Transporter α-β.Cellular and molecular gastroenterology and hepatology · 2018Article
- Full and Partial Agonism of a Designed Enzyme Switch.ACS synthetic biology · 2016Article
- The Research and Applications of Quantum Dots as Nano-Carriers for Targeted Drug Delivery and Cancer Therapy.Nanoscale research letters · 2016Review
- Article
- Real-time functional characterization of cationic amino acid transporters using a new FRET sensor.Pflugers Archiv : European journal of physiology · 2016Article
- Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor.Journal of visualized experiments : JoVE · 2016Article
- Synthetic biology devices for in vitro and in vivo diagnostics.Proceedings of the National Academy of Sciences of the United States of America · 2015Review
- A Modular, DNA-Based Beacon for Single-Step Fluorescence Detection of Antibodies and Other Proteins.Angewandte Chemie (International ed. in English) · 2015Article
- Engineering genetically encoded FRET sensors.Sensors (Basel, Switzerland) · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Proteins that switch between distinct conformational states are ideal to monitor and control molecular processes within the complexity of biological systems. Inspired by the modular architecture of natural signalling proteins, our group explores generic design strategies for the construction of FRET-based sensor proteins and other protein switches. In the present article, I show that designing FRET sensors based on mutually exclusive domain interactions provides a robust method to engineer sensors with predictable properties and an inherently large change in emission ratio. The modularity of this approach should make it easily transferable to other applications of protein switches in fields ranging from synthetic biology, optogenetics and molecular diagnostics.
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Registered trials
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