ArticleJournal of the American Chemical Society2013
Rational design of a colorimetric pH sensor from a soluble retinoic acid chaperone.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Photoisomerization detected in a fully wavelength-tunable rhodopsin mimic system.Acta crystallographica. Section D, Structural biology · 2026Article
- Advancements in Gene Delivery using Nucleic Acid Loaded Nanoparticles for Region Specific Delivery in Alzheimer's Disease.Molecular neurobiology · 2026Review
- Ligand protonation leads to highly fluorescent boronium cations.Chemical science · 2025Article
- Regulation of Absorption and Emission in a Protein/Fluorophore Complex.ACS chemical biology · 2024Article
- AlphaFill: enriching AlphaFold models with ligands and cofactors.Nature methods · 2023Article
- Excited-state dynamics of all-trans protonated retinal Schiff base in CRABPII-based rhodopsin mimics.Biophysical journal · 2022Article
- Design of Large Stokes Shift Fluorescent Proteins Based on Excited State Proton Transfer of an Engineered Photobase.Journal of the American Chemical Society · 2021Article
- Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.Molecules (Basel, Switzerland) · 2021Review
- Structure-functional relationship of cellular retinoic acid-binding proteins I and II interacting with natural and synthetic ligands.Acta crystallographica. Section D, Structural biology · 2021Article
- Engineering of a Red Fluorogenic Protein/Merocyanine Complex for Live-Cell Imaging.Chembiochem : a European journal of chemical biology · 2020Article
- Selective Colorimetric "Turn-On" Probe for Efficient Engineering of Iminium Biocatalysis.ACS omega · 2020Article
- Engineering the hCRBPII Domain-Swapped Dimer into a New Class of Protein Switches.Journal of the American Chemical Society · 2019Article
- Mimicking Microbial Rhodopsin Isomerization in a Single Crystal.Journal of the American Chemical Society · 2019Article
- A Genetically Encoded Ratiometric pH Probe: Wavelength Regulation-Inspired Design of pH Indicators.Chembiochem : a European journal of chemical biology · 2018Article
- Free-Energy-Based Protein Design: Re-Engineering Cellular Retinoic Acid Binding Protein II Assisted by the Moveable-Type Approach.Journal of the American Chemical Society · 2018Article
- Shedding light on biology of bacterial cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Domain-Swapped Dimers of Intracellular Lipid-Binding Proteins: Evidence for Ordered Folding Intermediates.Structure (London, England : 1993) · 2016Article
- A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.Journal of the American Chemical Society · 2016Article
- Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue.Journal of the American Chemical Society · 2016Article
- Light-Activated Reversible Imine Isomerization: Towards a Photochromic Protein Switch.Chembiochem : a European journal of chemical biology · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Reengineering of cellular retinoic acid binding protein II (CRABPII) to be capable of binding retinal as a protonated Schiff base is described. Through rational alterations of the binding pocket, electrostatic perturbations of the embedded retinylidene chromophore that favor delocalization of the iminium charge lead to exquisite control in the regulation of chromophoric absorption properties, spanning the visible spectrum (474-640 nm). The pKa of the retinylidene protonated Schiff base was modulated from 2.4 to 8.1, giving rise to a set of proteins of varying colors and pH sensitivities. These proteins were used to demonstrate a concentration-independent, ratiometric pH sensor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.