ArticleSpringerPlus2013
Hyperglycaemia and oxidative stress upregulate HSP60 & HSP70 expression in HeLa cells.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Effects of Chromium Yeast Supplementation on SerumVeterinary sciences · 2025Article
- Immunomodulatory Tissue Factors in the Gallbladder Walls of Pediatric Patients with Chronic Calculous Cholecystitis.Children (Basel, Switzerland) · 2025Article
- Exploring the Role of Hsp60 in Alzheimer's Disease and Type 2 Diabetes: Suggestion for Common Drug Targeting.International journal of molecular sciences · 2023Review
- Ectopic Lipid Accumulation Correlates with Cellular Stress in Rabbit Blastocysts from Diabetic Mothers.International journal of molecular sciences · 2023Article
- Serum Level of Heat Shock Protein 70 in Patients with Type 2 Diabetes Mellitus in Basrah, Iraq.Archives of Razi Institute · 2022Article
- Saturated fat ingestion stimulates proatherogenic inflammation in polycystic ovary syndrome.American journal of physiology. Endocrinology and metabolism · 2021Article
- HSP70 as a biomarker of the thin threshold between benefit and injury due to physical exercise when exposed to air pollution.Cell stress & chaperones · 2021Review
- Heat shock proteins with an emphasis on HSP 60.Molecular biology reports · 2021Review
- Molecular Chaperones: Molecular Assembly Line Brings Metabolism and Immunity in Shape.Metabolites · 2020Review
- Secondary Metabolites from the Culture of the Marine-derived FungusMarine drugs · 2020Article
- Role of Mitochondrial Stress Protein HSP60 in Diabetes-Induced Neuroinflammation.Mediators of inflammation · 2020Review
- Mitochondrial Heat Shock Response Induced by Ectromelia Virus is Accompanied by Reduced Apoptotic Potential in Murine L929 Fibroblasts.Archivum immunologiae et therapiae experimentalis · 2019Article
- Heat Shock Proteins as a Potential Therapeutic Target in the Treatment of Gestational Diabetes Mellitus: What We Know so Far.International journal of molecular sciences · 2018Review
- Correlation between heat shock proteins, adiponectin, and T lymphocyte cytokine expression in type 2 diabetics.Cell stress & chaperones · 2018Article
- Endothelial TNF-α induction by Hsp60 secreted from THP-1 monocytes exposed to hyperglycaemic conditions.Cell stress & chaperones · 2018Article
- Article
- 17β-Estradiol on the Expression of G-Protein Coupled Estrogen Receptor (GPER/GPR30) Mitophagy, and the PI3K/Akt Signaling Pathway in ATDC5 Chondrocytes In Vitro.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.Scientific reports · 2017Article
- Effects of Marine Oils, Digested with Human Fluids, on Cellular Viability and Stress Protein Expression in Human Intestinal Caco-2 Cells.Nutrients · 2017Article
- The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome.Free radical biology & medicine · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat Shock Proteins 60 & 70 (HSP60 & HSP70) are intracellular protein that has been shown to be present at elevated levels in systemic circulation in Type 2 Diabetes mellitus (T2DM) patients. Conditions that lead to its secretion, and the mechanism of its translocation from cells, have not yet been defined. The aim of this study was to determine if specific cell stressors associated with T2DM, namely hyperglycaemia and oxidative stress, result in the upregulation of HSP60 in human cells in vitro. Human HeLa cells were grown in media supplemented with 100 mM glucose, 200 μM hydrogen peroxide (H2O2), and 50 μM sodium azide. Initially, the effect of these treatments on cell growth rate was examined, with each treatment significantly inhibiting growth rate. LDH and MTT assays were also used to successfully demonstrate that these treatments do not significantly increase cell lysis, but do significantly impair mitochondrial dehydrogenase activity. To confirm this mitochondria specific form of inhibition, DCFDA assay were used to investigate any increases in intracellular reactive oxygen species (ROS) generation. All three treatments resulted in significantly increased ROS generation, with greater ROS production occurring with a greater exposure time. Interestingly, when the protein levels of HSP60 and HSP70 were measured after 3 and 7 days of exposure of the HeLa cells to 100 mM glucose, 200 μM H2O2, and 50 μM sodium azide significant induction of these two molecular stress proteins were observed ranging from 2.43-5.08 fold compared to untreated control cells.
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