ArticlePloS one2013
Sequence recombination and conservation of Varroa destructor virus-1 and deformed wing virus in field collected honey bees (Apis mellifera).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed.
- Opposite seasonal and spatial dynamics of DWV-A and DWV-B suggest distinct transmission pathways in managed honey bees (Apis mellifera L.) colonies.Scientific reports · 2026Article
- Bumble Bees and Honey Bees on Islands Harbour Reduced Viral Species Richness, Yet Honey Bee Populations Are Dominated by a Deformed Wing Virus Recombinant.Molecular ecology · 2025Article
- Parasitic Mites of Honey Bees (Apis Spp.): A Detailed Review of Varroa destructor in Parasitism, Pathogen Transmission and its Management.Acta parasitologica · 2025Review
- Superinfection Exclusion in Neotropical Honey Bees May Block DWV-B, an Emerging Infectious Disease Variant of Deformed Wing Virus.Evolutionary applications · 2025Article
- Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies.Scientific reports · 2025Article
- Origins, diversity, and adaptive evolution of DWV in the honey bees of the Azores: the impact of the invasive miteVirus evolution · 2024Article
- Virus Prevalence in Egg Samples Collected from Naturally Selected and Traditionally Managed Honey Bee Colonies across Europe.Viruses · 2022Article
- Bee-safe peptidomimetic acaricides achieved by comparative genomics.Scientific reports · 2022Article
- Epidemiology of a major honey bee pathogen, deformed wing virus: potential worldwide replacement of genotype A by genotype B.International journal for parasitology. Parasites and wildlife · 2022Article
- Article
- Dynamic evolution in the key honey bee pathogen deformed wing virus: Novel insights into virulence and competition using reverse genetics.PLoS biology · 2019Article
- Prevalence and population genetics of the emerging honey bee pathogen DWV in Chinese apiculture.Scientific reports · 2019Article
- Knock-on community impacts of a novel vector: spillover of emerging DWV-B from Varroa-infested honeybees to wild bumblebees.Ecology letters · 2019Article
- Article
- Characterization of viral RNA splicing using whole-transcriptome datasets from host species.Scientific reports · 2018Article
- Article
- ATP-sensitive inwardly rectifying potassium channel regulation of viral infections in honey bees.Scientific reports · 2017Article
- The virulent, emerging genotype B of Deformed wing virus is closely linked to overwinter honeybee worker loss.Scientific reports · 2017Article
- Evidence for positive selection and recombination hotspots in Deformed wing virus (DWV).Scientific reports · 2017Article
- Elevated virulence of an emerging viral genotype as a driver of honeybee loss.Proceedings. Biological sciences · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We sequenced small (s) RNAs from field collected honeybees (Apis mellifera) and bumblebees (Bombuspascuorum) using the Illumina technology. The sRNA reads were assembled and resulting contigs were used to search for virus homologues in GenBank. Matches with Varroadestructor virus-1 (VDV1) and Deformed wing virus (DWV) genomic sequences were obtained for A. mellifera but not B. pascuorum. Further analyses suggested that the prevalent virus population was composed of VDV-1 and a chimera of 5'-DWV-VDV1-DWV-3'. The recombination junctions in the chimera genomes were confirmed by using RT-PCR, cDNA cloning and Sanger sequencing. We then focused on conserved short fragments (CSF, size > 25 nt) in the virus genomes by using GenBank sequences and the deep sequencing data obtained in this study. The majority of CSF sites confirmed conservation at both between-species (GenBank sequences) and within-population (dataset of this study) levels. However, conserved nucleotide positions in the GenBank sequences might be variable at the within-population level. High mutation rates (Pi>10%) were observed at a number of sites using the deep sequencing data, suggesting that sequence conservation might not always be maintained at the population level. Virus-host interactions and strategies for developing RNAi treatments against VDV1/DWV infections are discussed.
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