ArticlePloS one2013
Temporal dynamics of abundance and composition of nitrogen-fixing communities across agricultural soils.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Stress-resilient endospore-forming diazotrophs from red lateritic soils of India: functional profiling and first evidence of Priestia abyssalis and Bacillus gaemokensis as nitrogen-fixers with multifunctional PGPR traits.World journal of microbiology & biotechnology · 2026Article
- Introduction ofFrontiers in microbiology · 2025Article
- Soil nitrogen-related functional genes undergo abundance changes during vegetation degradation in a Qinghai-Tibet Plateau wet meadow.Applied and environmental microbiology · 2024Article
- Microbial species pool-mediated diazotrophic community assembly in crop microbiomes during plant development.mSystems · 2024Article
- Interactions between halotolerant nitrogen-fixing bacteria and arbuscular mycorrhizal fungi under saline stress.Frontiers in microbiology · 2024Article
- Nitrogen fixation by diverse diazotrophic communities can support population growth of arboreal ants.BMC biology · 2022Article
- Diverse key nitrogen cycling genes nifH, nirS and nosZ associated with Pichavaram mangrove rhizospheres as revealed by culture-dependent and culture-independent analyses.Archives of microbiology · 2022Article
- Nodule-associated diazotrophic community succession is driven by developmental phases combined with microhabitat ofFrontiers in microbiology · 2022Article
- Temporal dynamics of total and active root-associated diazotrophic communities in field-grown rice.Frontiers in microbiology · 2022Article
- Community Composition and Co-Occurrence Patterns of Diazotrophs along a Soil Profile in Paddy Fields of Three Soil Types in China.Microbial ecology · 2021Article
- Seasonal and long-term effects of nutrient additions and liming on the nifH gene in cerrado soils under native vegetation.iScience · 2021Article
- Article
- Impact of Intercropping on the Diazotrophic Community in the Soils of Continuous Cucumber Cropping Systems.Frontiers in microbiology · 2021Article
- Article
- Nitrogen Fertilization and Native CFrontiers in microbiology · 2021Article
- DiazotrophicApplied and environmental microbiology · 2020Article
- Soil Bacterial Community Response and Nitrogen Cycling Variations Associated with Subalpine Meadow Degradation on the Loess Plateau, China.Applied and environmental microbiology · 2020Article
- Article
- Changes of diazotrophic communities in response to cropping systems in a Mollisol of Northeast China.PeerJ · 2020Article
- Ridge Tillage Improves Soil Properties, Sustains Diazotrophic Communities, and Enhances Extensively Cooperative Interactions Among Diazotrophs in a Clay Loam Soil.Frontiers in microbiology · 2020Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite the fact that the fixation of nitrogen is one of the most significant nutrient processes in the terrestrial ecosystem, a thorough study of the spatial and temporal patterns in the abundance and distribution of N-fixing communities has been missing so far. METHODOLOGY/PRINCIPAL
findingsIn order to understand the dynamics of diazotrophic communities and their resilience to external changes, we quantified the abundance and characterized the bacterial community structures based on the nifH gene, using real-time PCR, PCR-DGGE and 454-pyrosequencing, across four representative Dutch soils during one growing season. In general, higher nifH gene copy numbers were observed in soils with higher pH than in those with lower pH, but lower numbers were related to increased nitrate and ammonium levels. Results from nifH gene pyrosequencing confirmed the observed PCR-DGGE patterns, which indicated that the N fixers are highly dynamic across time, shifting around 60%. Forward selection on CCA analysis identified N availability as the main driver of these variations, as well as of the evenness of the communities, leading to very unequal communities. Moreover, deep sequencing of the nifH gene revealed that sandy soils (B and D) had the lowest percentage of shared OTUs across time, compared with clayey soils (G and K), indicating the presence of a community under constant change. Cosmopolitan nifH species (present throughout the season) were affiliated with Bradyrhizobium, Azospirillum and Methylocistis, whereas other species increased their abundances progressively over time, when appropriate conditions were met, as was notably the case for Paenibacilus and Burkholderia.
conclusionsOur study provides the first in-depth pyrosequencing analysis of the N-fixing community at both spatial and temporal scales, providing insights into the cosmopolitan and specific portions of the nitrogen fixing bacterial communities in soil.
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