ArticlePloS one2013
Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 76 citations in OpenAlex.
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
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- Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata.Scientific reports · 2022Article
- Reference genes identification for qRT-PCR normalization of gene expression analysis inFrontiers in plant science · 2022Article
- The amino acid permease (AAP) genes CsAAP2A and SlAAP5A/B are required for oomycete susceptibility in cucumber and tomato.Molecular plant pathology · 2021Article
- Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions.Scientific reports · 2021Article
- Selection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis.BMC plant biology · 2020Article
- Critical enzymes for biosynthesis of cucurbitacin derivatives in watermelon and their biological significance.Communications biology · 2020Article
- Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf.Molecular biology reports · 2020Article
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- Map-Based Functional Analysis of theInternational journal of molecular sciences · 2019Article
- Root ABA and HPlant direct · 2019Article
- Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune.BMC molecular biology · 2019Article
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- The overexpression of cucumber (Cucumis sativus L.) genes that encode the branched-chain amino acid transferase modulate flowering time in Arabidopsis thaliana.Plant cell reports · 2019Article
- Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar 'Huangguogan'.PloS one · 2019Article
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- Plant reference genes for development and stress response studies.Journal of biosciences · 2018Review
- Genome-wide identification of new reference genes for RT-qPCR normalization in CGMMV-infectedPeerJ · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, nitrogen is the most important nutritional factor limiting the yield of cultivated crops. Since nitrogen is essential for synthesis of nucleotides, amino acids and proteins, studies on gene expression in plants cultivated under different nitrogen availability require particularly careful selection of suitable reference genes which are not affected by nitrogen limitation. Therefore, the objective of this study was to select the most reliable reference genes for qPCR analysis of target cucumber genes under varying nitrogen source and availability. Among twelve candidate cucumber genes used in this study, five are highly homologous to the commonly used internal controls, whereas seven novel candidates were previously identified through the query of the cucumber genome. The expression of putative reference genes and the target CsNRT1.1 gene was analyzed in roots, stems and leaves of cucumbers grown under nitrogen deprivation, varying nitrate availability or different sources of nitrogen (glutamate, glutamine or NH3). The stability of candidate genes expression significantly varied depending on the tissue type and nitrogen supply. However, in most of the outputs genes encoding CACS, TIP41, F-box protein and EFα proved to be the most suitable for normalization of CsNRT1.1 expression. In addition, our results suggest the inclusion of 3 or 4 references to obtain highly reliable results of target genes expression in all cucumber organs under nitrogen-related stress.
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