ArticlePloS one2013
Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Prevalence and Clinical Impact of Respiratory Viral Infections from the STOP2 Study of Cystic Fibrosis Pulmonary Exacerbations.Annals of the American Thoracic Society · 2024Trial
- Interference Between Respiratory Syncytial Virus and Human Rhinovirus Infection in Infancy.The Journal of infectious diseases · 2017Trial
- Epidemiological Characterization of Respiratory Pathogens Using the Multiplex PCR FilmArray™ Respiratory Panel.Diagnostics (Basel, Switzerland) · 2024Article
- Optical reflectometric measurement of SARS-CoV-2 (COVID-19) RNA based on cationic cysteamine-capped gold nanoparticles.Optics and laser technology · 2023Article
- A CRISPR-Cas12a-Based platform for ultrasensitive, rapid, and highly specific detection ofFrontiers in bioengineering and biotechnology · 2023Article
- Overnutrition, Nasopharyngeal Pathogenic Bacteria and Proinflammatory Cytokines in Infants with Viral Lower Respiratory Tract Infections.International journal of environmental research and public health · 2022Article
- A fulminant pneumonia due to Mycoplasma pneumoniae - Case report and literature review.IDCases · 2022Article
- Sputum versus nasopharyngeal samples for the molecular diagnosis of respiratory viral infection in cystic fibrosis: A pilot study.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2021Article
- Respiratory microbes detected in hospitalized adults with acute respiratory infections: associations between influenza A(H1N1)pdm09 virus and intensive care unit admission or fatal outcome in Vietnam (2015-2017).BMC infectious diseases · 2021Article
- Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants.Advances in virology · 2020Article
- Rapid testing for respiratory syncytial virus in a resource-limited paediatric intensive care setting.African journal of laboratory medicine · 2020Article
- Clinical characteristics of the lower respiratory tract infection caused by a single infection or coinfection of the human parainfluenza virus in children.Journal of medical virology · 2019Article
- A survey on incidence and management of adenovirus infection after allogeneic HSCT.Bone marrow transplantation · 2019Article
- Viral pathogens associated with acute respiratory illness in hospitalized adults and elderly from Zagreb, Croatia, 2016 to 2018.Journal of medical virology · 2019Article
- Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China.Biomedical and environmental sciences : BES · 2019Article
- Evaluation of the ELITe InGenius PCR Platform for Detection of Mycoplasma pneumoniae.Journal of clinical microbiology · 2019Article
- Influence of viral load in the outcome of hospitalized patients with influenza virus infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Observational
- Comparison of the Panther Fusion and Allplex assays for the detection of respiratory viruses in clinical samples.PloS one · 2019Article
- Innovative DendrisChipsDiagnostics (Basel, Switzerland) · 2018Article
- Comparison of the Biofire FilmArray Respiratory Panel, Seegene AnyplexII RV16, and Argene for the detection of respiratory viruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular diagnosis of respiratory infection can be performed using different commercial multiplex-based PCR kits whose performances have been previously compared individually to those of conventional techniques. This study compared the practicability and the diagnostic performances of six CE-marked kits available in 2011 on the French market, including 2 detecting viruses and atypical bacteria (from Pathofinder and Seegene companies) and 4 detecting only viruses (from Abbott, Genomica, Qiagen and Seegene companies). The respective sensitivity, specificity, accuracy and agreement of each multiplex technique were calculated by comparison to commercial duplex PCR tests (Argene/bioMérieux) used as gold standard. Eighty-eight respiratory specimens with no pathogen (n = 11), single infections (n = 33) or co-infections (n = 44) were selected to cover 9 viruses or groups of viruses and 3 atypical bacteria. All samples were extracted using the NUCLISENS® easyMAG™ instrument (bioMérieux). The overall sensitivity ranged from 56.25% to 91.67% for viruses and was below 50% with both tests for bacteria. The overall specificity was excellent (>94% for all pathogens). For each tested kit, the overall agreement with the reference test was strong for viruses (kappa test >0.60) and moderate for bacteria. After the extraction step, the hands-on time varied from 50 min to 2h30 and the complete results were available in 2h30 to 9 h. The spectrum of tested agents and the technology used to reveal the PCR products as well as the laboratory organization are determinant for the selection of a kit.
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