ArticlePLoS biology2013
A bipartite molecular module controls cell death activation in the Basal cell lineage of plant embryos.
Article in PLoS biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 88 citations in OpenAlex.
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- NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana.The New phytologist · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- The mysterious dialogue between the embryo, endosperm, and seed coat, and its implications for seed traits.Journal of experimental botany · 2026Review
- ATG5-HSP90.2-mediated micromitophagy as a cytological basis for maternal inheritance of plant mitochondria.Nature plants · 2026Article
- Identification and expression analysis of papain-like cysteine proteases gene family and response to B. cinerea stress in F. vesca.BMC plant biology · 2025Article
- RD21-like proteases: key effector hubs in plant-pathogen interactions.Journal of experimental botany · 2025Review
- Circular cell clusters and calcium oxalate crystals: critical players in Solanaceae anther dehiscence.Planta · 2025Review
- Autophagy-mediated degradation of integumentary tapetum is critical for embryo pattern formation.Nature communications · 2024Article
- Morphological and anatomical changes during dormancy break of the seeds ofPlant signaling & behavior · 2023Article
- Secretome analysis revealed that cell wall remodeling and starch catabolism underlie the early stages of somatic embryogenesis inFrontiers in plant science · 2023Article
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- Molecular Aspects of Seed Development Controlled by Gibberellins and Abscisic Acids.International journal of molecular sciences · 2022Review
- SYP72 interacts with the mechanosensitive channel MSL8 to protect pollen from hypoosmotic shock during hydration.Nature communications · 2022Article
- Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Detection of Embryonic Suspensor Cell Death by Whole-Mount TUNEL Assay in Tobacco.Plants (Basel, Switzerland) · 2020Article
- Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos.Nature communications · 2020Article
- Manual Isolation of Living Early Embryos from Tobacco Seeds.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Maternal control of suspensor programmed cell death via gibberellin signaling.Nature communications · 2019Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant zygote divides asymmetrically into an apical cell that develops into the embryo proper and a basal cell that generates the suspensor, a vital organ functioning as a conduit of nutrients and growth factors to the embryo proper. After the suspensor has fulfilled its function, it is removed by programmed cell death (PCD) at the late stages of embryogenesis. The molecular trigger of this PCD is unknown. Here we use tobacco (Nicotiana tabacum) embryogenesis as a model system to demonstrate that the mechanism triggering suspensor PCD is based on the antagonistic action of two proteins: a protease inhibitor, cystatin NtCYS, and its target, cathepsin H-like protease NtCP14. NtCYS is expressed in the basal cell of the proembryo, where encoded cystatin binds to and inhibits NtCP14, thereby preventing precocious onset of PCD. The anti-cell death effect of NtCYS is transcriptionally regulated and is repressed at the 32-celled embryo stage, leading to increased NtCP14 activity and initiation of PCD. Silencing of NtCYS or overexpression of NtCP14 induces precocious cell death in the basal cell lineage causing embryonic arrest and seed abortion. Conversely, overexpression of NtCYS or silencing of NtCP14 leads to profound delay of suspensor PCD. Our results demonstrate that NtCYS-mediated inhibition of NtCP14 protease acts as a bipartite molecular module to control initiation of PCD in the basal cell lineage of plant embryos.
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