ArticleEukaryotic cell2014
Evidence of intraflagellar transport and apical complex formation in a free-living relative of the apicomplexa.
Article in Eukaryotic cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- SeparatingBio-protocol · 2026Article
- Transfection of the free-living alga Chromera velia enables direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondii.Journal of cell science · 2026Article
- Transfection of the free-living algabioRxiv : the preprint server for biology · 2025Article
- Adaptations and metabolic evolution of myzozoan protists across diverse lifestyles and environments.Microbiology and molecular biology reviews : MMBR · 2024Review
- The initiation and early development of apical-basal polarity in Toxoplasma gondii.Journal of cell science · 2024Article
- The initiation and early development of the tubulin-containing cytoskeleton in the human parasiteMolecular biology of the cell · 2024Article
- Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins.Nature communications · 2024Article
- Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery.Nature communications · 2023Article
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- Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure inMolecular biology of the cell · 2020Article
- A doublecortin-domain protein of Toxoplasma and its orthologues bind to and modify the structure and organization of tubulin polymers.BMC molecular and cell biology · 2020Article
- A conserved ankyrin repeat-containing protein regulates conoid stability, motility and cell invasion in Toxoplasma gondii.Nature communications · 2017Article
- Loss of a doublecortin (DCX)-domain protein causes structural defects in a tubulin-based organelle of Toxoplasma gondii and impairs host-cell invasion.Molecular biology of the cell · 2017Article
- SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector.Scientific reports · 2016Article
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- Targeting Toxoplasma tubules: tubulin, microtubules, and associated proteins in a human pathogen.Eukaryotic cell · 2015Review
- The apical complex provides a regulated gateway for secretion of invasion factors in Toxoplasma.PLoS pathogens · 2014Article
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Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since its first description, Chromera velia has attracted keen interest as the closest free-living relative of parasitic Apicomplexa. The life cycle of this unicellular alga is complex and involves a motile biflagellate form. Flagella are thought to be formed in the cytoplasm, a rare phenomenon shared with Plasmodium in which the canonical mode of flagellar assembly, intraflagellar transport, is dispensed with. Here we demonstrate the expression of intraflagellar transport components in C. velia, answering the question of whether this organism has the potential to assemble flagella via the canonical route. We have developed and characterized a culturing protocol that favors the generation of flagellate forms. From this, we have determined a marked shift in the mode of daughter cell production from two to four daughter cells per division as a function of time after passage. We conduct an ultrastructural examination of the C. velia flagellate form by using serial TEM and show that flagellar biogenesis in C. velia occurs prior to cytokinesis. We demonstrate a close association of the flagellar apparatus with a complex system of apical structures, including a micropore, a conoid, and a complex endomembrane system reminiscent of the apical complex of parasitic apicomplexans. Recent work has begun to elucidate the possible flagellar origins of the apical complex, and we show that in C. velia these structures are contemporaneous within a single cell and share multiple connections. We propose that C. velia therefore represents a vital piece in the puzzle of the origins of the apical complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.