ArticleMethods in molecular biology (Clifton, N.J.)2014
Protein fragment bimolecular fluorescence complementation analyses for the in vivo study of protein-protein interactions and cellular protein complex localizations.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Deciphering cassava brown streak virus infection in cassava through VPg mediated host protein interactions.Archives of virology · 2026Article
- Catch & Release-rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach.The Plant journal : for cell and molecular biology · 2025Article
- The bZIP transcription factor AREB3 mediates FT signalling and floral transition at the Arabidopsis shoot apical meristem.PLoS genetics · 2023Article
- Detecting Protein-Protein Interactions Using Bimolecular Fluorescence Complementation (BiFC) and Luciferase Complementation Assays (LCA).Methods in molecular biology (Clifton, N.J.) · 2023Article
- A novel calmodulin-interacting Domain of Unknown Function 506 protein represses root hair elongation in Arabidopsis.Plant, cell & environment · 2022Article
- A phosphorus-limitation induced, functionally conserved DUF506 protein is a repressor of root hair elongation in plants.The New phytologist · 2022Article
- Two plastid POLLUX ion channel-like proteins are required for stress-triggered stromal Ca2+release.Plant physiology · 2021Article
- Recent advances in cellular biosensor technology to investigate tau oligomerization.Bioengineering & translational medicine · 2021Review
- Protein complex formation in methionine chain-elongation and leucine biosynthesis.Scientific reports · 2021Article
- Photosynthesis in Arabidopsis Is Unaffected by the Function of the Vacuolar KPlant physiology · 2019Article
- Surveying the landscape of optogenetic methods for detection of protein-protein interactions.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast.Nature communications · 2016Article
- Lighting the Way to Protein-Protein Interactions: Recommendations on Best Practices for Bimolecular Fluorescence Complementation Analyses.The Plant cell · 2016Article
- HSP90 regulates temperature-dependent seedling growth in Arabidopsis by stabilizing the auxin co-receptor F-box protein TIR1.Nature communications · 2016Article
- Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses.Plant physiology · 2015Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The analyses of protein-protein interactions are crucial for understanding cellular processes including signal transduction, protein trafficking, and movement. Protein fragment complementation assays are based on the reconstitution of protein function when non-active protein fragments are brought together by interacting proteins that were genetically fused to these protein fragments. Bimolecular fluorescence complementation (BiFC) relies on the reconstitution of fluorescent proteins and enables both the analysis of protein-protein interactions and the visualization of protein complex formations in vivo. Transient expression of proteins is a convenient approach to study protein functions in planta or in other organisms and minimizes the need for time-consuming generation of stably expressing transgenic organisms. Here we describe protocols for BiFC analyses in Nicotiana benthamiana and Arabidopsis thaliana leaves transiently transformed by Agrobacterium infiltration. Further, we discuss different BiFC applications and provide examples for proper BiFC analyses in planta.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.