ArticleMolecular biology reports2013
Validation of reference genes for quantitative gene expression studies in Volvox carteri using real-time RT-PCR.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Cell Type-Specific Promoters ofGenes · 2023Article
- Molecular and cellular dynamics of early embryonic cell divisions in Volvox carteri.The Plant cell · 2022Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Simplified Transformation ofGenes · 2019Article
- Ice-Binding Proteins in a Chrysophycean Snow Alga: Acquisition of an Essential Gene by Horizontal Gene Transfer.Frontiers in microbiology · 2019Article
- Two-component cyclase opsins of green algae are ATP-dependent and light-inhibited guanylyl cyclases.BMC biology · 2018Article
- Additive effect of calcium depletion and low resource quality on Gammarus fossarum (Crustacea, Amphipoda) life history traits.Environmental science and pollution research international · 2018Article
- Whole transcriptome RNA-Seq analysis reveals extensive cell type-specific compartmentalization in Volvox carteri.BMC biology · 2017Article
- Article
- Transcriptome analysis reveals global regulation in response to CO2 supplementation in oleaginous microalga Coccomyxa subellipsoidea C-169.Biotechnology for biofuels · 2016Article
- Article
- Identification of the valid reference genes for quantitative RT-PCR in annual ryegrass (Lolium multiflorum) under salt stress.Molecules (Basel, Switzerland) · 2015Article
- The inducible nitA promoter provides a powerful molecular switch for transgene expression in Volvox carteri.BMC biotechnology · 2015Article
- Transcriptional analysis of Volvox photoreceptors suggests the existence of different cell-type specific light-signaling pathways.Current genetics · 2015Article
- Screening for Suitable Reference Genes for Quantitative Real-Time PCR in Heterosigma akashiwo (Raphidophyceae).PloS one · 2015Article
- Laccase Production and Differential Transcription of Laccase Genes in Cerrena sp. in Response to Metal Ions, Aromatic Compounds, and Nutrients.Frontiers in microbiology · 2015Article
- Cell-type specific photoreceptors and light signaling pathways in the multicellular green alga Volvox carteri and their potential role in cellular differentiation.Plant signaling & behavior · 2015Article
- Characterization of reference genes for RT-qPCR in the desert moss Syntrichia caninervis in response to abiotic stress and desiccation/rehydration.Frontiers in plant science · 2015Article
- Cell-type specific light-mediated transcript regulation in the multicellular alga Volvox carteri.BMC genomics · 2014Article
- Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) is a sensitive technique for analysis of gene expression under a wide diversity of biological conditions. However, the identification of suitable reference genes is a critical factor for analysis of gene expression data. To determine potential reference genes for normalization of qRT-PCR data in the green alga Volvox carteri, the transcript levels of ten candidate reference genes were measured by qRT-PCR in three experimental sample pools containing different developmental stages, cell types and stress treatments. The expression stability of the candidate reference genes was then calculated using the algorithms geNorm, NormFinder and BestKeeper. The genes for 18S ribosomal RNA (18S) and eukaryotic translation elongation factor 1α2 (eef1) turned out to have the most stable expression levels among the samples both from different developmental stages and different stress treatments. The genes for the ribosomal protein L23 (rpl23) and the TATA-box binding protein (tbpA) showed equivalent transcript levels in the comparison of different cell types, and therefore, can be used as reference genes for cell-type specific gene expression analysis. Our results indicate that more than one reference gene is required for accurate normalization of qRT-PCRs in V. carteri. The reference genes in our study show a much better performance than the housekeeping genes used as a reference in previous studies.
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