ArticleNucleic acids research2014
The tenacious recognition of yeast telomere sequence by Cdc13 is fully exerted by a single OB-fold domain.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 28 citations in OpenAlex.
- CST complex promotes second-strand synthesis in break-induced replication.Nature structural & molecular biology · 2026Article
- Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA.Nucleic acids research · 2024Article
- Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.Biochemistry · 2023Article
- The Thermodynamic Fingerprints of Ultra-Tight Nanobody-Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection.International journal of molecular sciences · 2023Article
- RPA-like single-stranded DNA-binding protein complexes including CST serve as specialized processivity factors for polymerases.Current opinion in structural biology · 2023Review
- A comprehensive map of hotspots of de novo telomere addition in Saccharomyces cerevisiae.Genetics · 2023Article
- A comprehensive map of hotspots of de novo telomere addition inbioRxiv : the preprint server for biology · 2023Article
- Molecular architecture and oligomerization of Candida glabrata Cdc13 underpin its telomeric DNA-binding and unfolding activity.Nucleic acids research · 2023Article
- Telomeres and Cancer.Life (Basel, Switzerland) · 2021Review
- When the Ends Justify the Means: Regulation of Telomere Addition at Double-Strand Breaks in Yeast.Frontiers in cell and developmental biology · 2021Review
- Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inThe Journal of biological chemistry · 2020Article
- Roles of OB-Fold Proteins in Replication Stress.Frontiers in cell and developmental biology · 2020Review
- Fine tuning the level of the Cdc13 telomere-capping protein for maximal chromosome stability performance.Current genetics · 2019Review
- Measuring Low-Picomolar Apparent Binding Affinities by Minigel Electrophoretic Mobility Shift.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nuclear import of Cdc13 limits chromosomal capping.Nucleic acids research · 2018Article
- Emerging roles of CST in maintaining genome stability and human disease.Frontiers in bioscience (Landmark edition) · 2018Review
- Rap1 and Cdc13 have complementary roles in preventing exonucleolytic degradation of telomere 5' ends.Scientific reports · 2017Article
- Human CST Prefers G-Rich but Not Necessarily Telomeric Sequences.Biochemistry · 2017Article
- Tying up the Ends: Plasticity in the Recognition of Single-Stranded DNA at Telomeres.Biochemistry · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cdc13, the telomere end-binding protein from Saccharomyces cerevisiae, is a multidomain protein that specifically binds telomeric single-stranded DNA (ssDNA) with exquisitely high affinity to coordinate telomere maintenance. Recent structural and genetic data have led to the proposal that Cdc13 is the paralog of RPA70 within a telomere-specific RPA complex. Our understanding of Cdc13 structure and biochemistry has been largely restricted to studies of individual domains, precluding analysis of how each domain influences the activity of the others. To better facilitate a comparison to RPA70, we evaluated the ssDNA binding of full-length S. cerevisiae Cdc13 to its minimal substrate, Tel11. We found that, unlike RPA70 and the other known telomere end-binding proteins, the core Cdc13 ssDNA-binding activity is wholly contained within a single tight-binding oligosaccharide/oligonucleotide/oligopeptide binding (OB)-fold. Because two OB-folds are implicated in dimerization, we also evaluated the relationship between dimerization and ssDNA-binding activity and found that the two activities are independent. We also find that Cdc13 binding exhibits positive cooperativity that is independent of dimerization. This study reveals that, while Cdc13 and RPA70 share similar domain topologies, the corresponding domains have evolved different and specialized functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.