ArticleJournal of visualized experiments : JoVE2013
A protocol for genetic induction and visualization of benign and invasive tumors in cephalic complexes of Drosophila melanogaster.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 citing papers in PubMed, 5 citations in OpenAlex.
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1 author at 1 institution in 1 country.
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Abstract
Drosophila has illuminated our understanding of the genetic basis of normal development and disease for the past several decades and today it continues to contribute immensely to our understanding of complex diseases (1-7). Progression of tumors from a benign to a metastatic state is a complex event (8) and has been modeled in Drosophila to help us better understand the genetic basis of this disease (9). Here I present a simple protocol to genetically induce, observe and then analyze the progression of tumors in Drosophila larvae. The tumor induction technique is based on the MARCM system (10) and exploits the cooperation between an activated oncogene, Ras(V12) and loss of cell polarity genes (scribbled, discs large and lethal giant larvae) to generate invasive tumors (9). I demonstrate how these tumors can be visualized in the intact larvae and then how these can be dissected out for further analysis. The simplified protocol presented here should make it possible for this technique to be utilized by investigators interested in understanding the role of a gene in tumor invasion.
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